Cloned (Comment) | Organism |
---|---|
gene pduO, recombinant overexpression of His-tagged wild-type and mutant PduOs in Escherichia coli | Limosilactobacillus reuteri |
Protein Variants | Comment | Organism |
---|---|---|
D35N | site-directed mutagenesis | Limosilactobacillus reuteri |
F112A | site-directed mutagenesis | Limosilactobacillus reuteri |
F112H | site-directed mutagenesis | Limosilactobacillus reuteri |
F187A | site-directed mutagenesis | Limosilactobacillus reuteri |
R128K | site-directed mutagenesis | Limosilactobacillus reuteri |
R132K | site-directed mutagenesis | Limosilactobacillus reuteri |
S159A | site-directed mutagenesis | Limosilactobacillus reuteri |
V186A | site-directed mutagenesis | Limosilactobacillus reuteri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | Limosilactobacillus reuteri | - |
triphosphate + adenosylcob(I)alamine | - |
? | |
ATP + cobinamide | Limosilactobacillus reuteri | - |
triphosphate + adenosylcobinamide | - |
? | |
additional information | Limosilactobacillus reuteri | The PduO-type ATP:corrinoid adenosyltransferase catalyzes the transfer of the adenosyl-group of ATP to Co1+-cobalamin and Co1+-cobinamide substrates to synthesize adenosylcobalamin and adenosylcobinamide, respectively | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | - |
gene pduO | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant PduOs from Escherichia coli by nickel affinity chromatography to over 99% homogeneity | Limosilactobacillus reuteri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 ATP + 2 cob(II)alamin + a reduced flavoprotein = 2 triphosphate + 2 adenosylcob(III)alamin + an oxidized flavoprotein | to overcome the thermodynamically challenging Co2+ -> Co1+ reduction, the enzyme drastically weakens the axial ligand-Co2+ bond so as to generate effectively four-coordinate Co2+-corrinoid species, mechanism, overview. The entire hydrophobic pocket below the corrin ring, and not just residue F112, is critical for the removal of the axial ligand from the cobalt center of the Co2+-corrinoids. Large role of the ATP-induced active-site conformational changes with respect to the formation of 4c Co(II)Cbl | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cob(I)alamin | - |
Limosilactobacillus reuteri | triphosphate + adenosylcob(I)alamine | - |
? | |
ATP + cobinamide | - |
Limosilactobacillus reuteri | triphosphate + adenosylcobinamide | - |
? | |
additional information | The PduO-type ATP:corrinoid adenosyltransferase catalyzes the transfer of the adenosyl-group of ATP to Co1+-cobalamin and Co1+-cobinamide substrates to synthesize adenosylcobalamin and adenosylcobinamide, respectively | Limosilactobacillus reuteri | ? | - |
? | |
additional information | to overcome the thermodynamically challenging Co2+ -> Co1+ reduction, the enzyme drastically weakens the axial ligand-Co2+ bond so as to generate effectively four-coordinate Co2+-corrinoid species, mechanism, overview. The entire hydrophobic pocket below the corrin ring, and not just residue F112, is critical for the removal of the axial ligand from the cobalt center of the Co2+-corrinoids. Large role of the ATP-induced active-site conformational changes with respect to the formation of 4c Co(II)Cbl | Limosilactobacillus reuteri | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PduO | - |
Limosilactobacillus reuteri |
PduO-type adenosine 5'-triphosphate:corrinoid adenosyltransferase | - |
Limosilactobacillus reuteri |
PduO-type ATP:corrinoid adenosyltransferase | - |
Limosilactobacillus reuteri |
General Information | Comment | Organism |
---|---|---|
additional information | active-site region of wild-type and mutant PduOs complexed with Co(II)Cbl and cosubstrate ATP, structure determination and modeling, overview | Limosilactobacillus reuteri |