Literature summary for 2.5.1.17 extracted from

Park, A.K.; Moon, J.H.; Lee, S.H.; Chi, Y.M.
Crystallization and preliminary X-ray crystallographic studies of a PduO-type ATP:cob(I)alamin adenosyltransferase from Bacillus cereus (2008), Acta Crystallogr. Sect. F, 64, 648-650.

Cloned(Commentary)

Cloned (Comment)	Organism
from genomic DNA in pET-28a for expression in Escherichia coli BL21 (DE3)	Bacillus cereus

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoenzyme and in complex with Mg2+/ATP, C-centred orthorhombic space group C222(1), unit cell parameters: a: 64.93 A, b: 137.08 A, c: 158.55 A, alpha, beta, gamma: 90°, one trimer in the asymmetric unit, sitting-drop combined with hanging-drop vapour-diffusion method: 13 mg/ml protein solution, precipitants: 1.55-1.6 M ammonium sulfate, 9-10% (v/v) dioxane (pH 6.5), for complex: 4 mM ATP and 4 mM Mg2+	Bacillus cereus

Crystallization (Comment)

Organism

apoenzyme and in complex with Mg2+/ATP, C-centred orthorhombic space group C222(1), unit cell parameters: a: 64.93 A, b: 137.08 A, c: 158.55 A, alpha, beta, gamma: 90°, one trimer in the asymmetric unit, sitting-drop combined with hanging-drop vapour-diffusion method: 13 mg/ml protein solution, precipitants: 1.55-1.6 M ammonium sulfate, 9-10% (v/v) dioxane (pH 6.5), for complex: 4 mM ATP and 4 mM Mg2+

Bacillus cereus

Organism

Organism	UniProt	Comment	Textmining
Bacillus cereus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-chelating HiTrap chromatography (elution with 1 M imidazole), HiLoad 16/60 Superdex 200 gel filtration chromatography (2 mM dithiothreitol), concentration to 13 mg/ml by ultracentrifugation	Bacillus cereus

Synonyms

Synonyms	Comment	Organism
PduO	-	Bacillus cereus
PduO enzyme	-	Bacillus cereus
PduO-type ATP:cob(I)alamin adenosyltransferase	-	Bacillus cereus

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Release 2023.1 (January 2023)