Cloned (Comment) | Organism |
---|---|
gene pduO, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Limosilactobacillus reuteri |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme in complex with its substrates, hanging drop vapour diffusion method, 20°C, 0.004 ml of 20 mg/ml protein in 10 mM Tris-HCl, pH 8.0, is mixed with 0.004 ml od precipitant solution containing 0.1 M HEPES, pH 8.5, 1.1 M ammonium sulfate, 2 mM ATP, 55 mM MgCl2, 165 mM NaCl, and 15 mM cob(I)alamin, 7 days, X-ray diffraction structure determination and analysis at 1.68 A resolution | Limosilactobacillus reuteri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
cob(I)alamin | - |
Limosilactobacillus reuteri | |
0.0022 | - |
ATP | - |
Limosilactobacillus reuteri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Limosilactobacillus reuteri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cob(I)alamin + ATP | Limosilactobacillus reuteri | adenosylation of the corrinoid ring of cob(I)alamin generates coenzyme B12, i.e. adenosylcobalamin or AdoCbl, an essential cofactor used by enzymes that catalyze intramolecular rearrangements, deaminations, dehydrations, reductions, and reductive dehalogenations | adenosylcobalamin + triphosphate | - |
? | |
cob(I)alamin + ATP | Limosilactobacillus reuteri CRL1098 | adenosylation of the corrinoid ring of cob(I)alamin generates coenzyme B12, i.e. adenosylcobalamin or AdoCbl, an essential cofactor used by enzymes that catalyze intramolecular rearrangements, deaminations, dehydrations, reductions, and reductive dehalogenations | adenosylcobalamin + triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | - |
gene pduO | - |
Limosilactobacillus reuteri CRL1098 | - |
gene pduO | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Limosilactobacillus reuteri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 ATP + 2 cob(II)alamin + a reduced flavoprotein = 2 triphosphate + 2 adenosylcob(III)alamin + an oxidized flavoprotein | active site and substrate binding sites and structures, molecular architecture | Limosilactobacillus reuteri | |
2 ATP + 2 cob(II)yrinic acid a,c-diamide + a reduced flavoprotein = 2 triphosphate + 2 adenosylcob(III)yrinic acid a,c-diamide + an oxidized flavoprotein | active site and substrate binding sites and structures, molecular architecture | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cob(I)alamin + ATP | adenosylation of the corrinoid ring of cob(I)alamin generates coenzyme B12, i.e. adenosylcobalamin or AdoCbl, an essential cofactor used by enzymes that catalyze intramolecular rearrangements, deaminations, dehydrations, reductions, and reductive dehalogenations | Limosilactobacillus reuteri | adenosylcobalamin + triphosphate | - |
? | |
cob(I)alamin + ATP | adenosylation of the corrinoid ring of cob(I)alamin, active site structure, ATP binding motif at the protein N terminus | Limosilactobacillus reuteri | adenosylcobalamin + triphosphate | - |
? | |
cob(I)alamin + ATP | adenosylation of the corrinoid ring of cob(I)alamin generates coenzyme B12, i.e. adenosylcobalamin or AdoCbl, an essential cofactor used by enzymes that catalyze intramolecular rearrangements, deaminations, dehydrations, reductions, and reductive dehalogenations | Limosilactobacillus reuteri CRL1098 | adenosylcobalamin + triphosphate | - |
? | |
cob(I)alamin + ATP | adenosylation of the corrinoid ring of cob(I)alamin, active site structure, ATP binding motif at the protein N terminus | Limosilactobacillus reuteri CRL1098 | adenosylcobalamin + triphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PduO | - |
Limosilactobacillus reuteri |
PduO-type ATP:Co(I)rrinoid adenosyltransferase | - |
Limosilactobacillus reuteri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Limosilactobacillus reuteri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
ATP | - |
Limosilactobacillus reuteri | |
0.025 | - |
cob(I)alamin | - |
Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Limosilactobacillus reuteri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | ATP binding motif at the protein N terminus | Limosilactobacillus reuteri |