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Literature summary for 2.5.1.17 extracted from

  • Johnson, C.L.; Buszko, M.L.; Bobik, T.A.
    Purification and initial characterization of the Salmonella enterica PduO ATP:Cob(I)alamin adenosyltransferase (2004), J. Bacteriol., 186, 7881-7887.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.17

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme in Escherichia coli in inclusion bodies, expression of truncated mutants in Escherichia coli Salmonella enterica

Protein Variants

Protein Variants Comment Organism
additional information construction of several truncated mutants, the activity decreases with truncation degree, remaining residues 1-90 are inactive, overview Salmonella enterica

Inhibitors

Inhibitors Comment Organism Structure
2'-deoxyadenosine weak inhibition Salmonella enterica
adenine weak inhibition Salmonella enterica
adenosine weak inhibition Salmonella enterica
ADP about 90% inhibition Salmonella enterica
Cd2+ complete inhibition Salmonella enterica
Cu2+ complete inhibition Salmonella enterica
additional information CTP, GTP, UTP, and AMP are no inhibitors Salmonella enterica
Ni2+ complete inhibition Salmonella enterica
Zn2+ complete inhibition Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Salmonella enterica
0.0045
-
 cob(I)alamin pH 8.0, 37°C, recombinant full length wild-type enzyme Salmonella enterica
0.0198
-
 ATP pH 8.0, 37°C, recombinant full length wild-type enzyme Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activity with divalent cations in descending order: Mg2+, Mn2+, Co2+ Salmonella enterica
Mg2+ required for activity, in absence of Mg2+ activity is reduced to 32% of maximum activity, activity with divalent cations in descending order: Mg2+, Mn2+, Co2+ Salmonella enterica
Mn2+ activity with divalent cations in descending order: Mg2+, Mn2+, Co2+ Salmonella enterica
additional information Ca2+ has no effect on enzyme activity Salmonella enterica
titanium citrate
-
 Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36812
-
 x * 36812, sequence calculation, full length enzyme Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cob(I)alamin Salmonella enterica final step in the conversion of vitamin B12 to coenzyme B12, the latter being required for degradation of 1,2-propanediol triphosphate + adenosylcob(III)alamin
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant solubilized enzyme from Escherichia coli 1.4fold to homogeneity, partial purification of recombinant truncated mutants, recombinant N-terminal domain to homogeneity Salmonella enterica

Renatured (Commentary)

Renatured (Comment) Organism
solubilization of wild-type enzyme and truncated enzyme forms from inclusion bodies after expression in Escherichia coli Salmonella enterica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activities of recombinant truncated enzyme versions Salmonella enterica
0.091
-
 purified recombinant full length wild-type enzyme, in absence of Mg2+ Salmonella enterica
0.262
-
 purified recombinant full length wild-type enzyme, in presence of 2.5 mM MgCl2 Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamin final step in the conversion of vitamin B12 to coenzyme B12, the latter being required for degradation of 1,2-propanediol Salmonella enterica triphosphate + adenosylcob(III)alamin
-
 ?
ATP + cobalamin enzyme is absolutely specific for ATP or dATP as adenosyl donors, ATP is the preferred adenosyl donor Salmonella enterica triphosphate + adenosylcob(III)alamin
-
 ?
dATP + cobalamin enzyme is absolutely specific for ATP or dATP as adenosyl donors, dATP results in 21% of the activity with ATP Salmonella enterica triphosphate + deoxyadenosylcob(III)alamin
-
 ?
additional information random or ordered ternary complex mechanism, the N-terminal domain is responsible for the catalytic activity and substrate binding and has similar biochemical properties and kinetic constants as the full-length enzyme Salmonella enterica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 36812, sequence calculation, full length enzyme Salmonella enterica
More approximate molecular weight of full length and truncated mutants Salmonella enterica

Synonyms

Synonyms Comment Organism
ATP:cob(I)alamin adenosyltransferase
-
 Salmonella enterica
PduO
-
 Salmonella enterica
PduO adenosyltransferase
-
 Salmonella enterica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Salmonella enterica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information recombinant truncated enzyme versions Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Salmonella enterica