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Literature summary for 2.5.1.17 extracted from

  • Suh, S.J.; Escalante-Semerena, J.C.
    Purification and initial characterization of the ATP:corrinoid adenosyltransferase encoded by the cobA gene of Salmonella typhimurium (1995), J. Bacteriol., 177, 921-925.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
DTT required to maintain activity Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
ATP pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.0052
-
cob(I)alamin pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
K+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Mg2+
-
Salmonella enterica subsp. enterica serovar Typhimurium
Mn2+
-
Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
2 * 25000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
42000
-
non-denaturing PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
structural gene cob A
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation followed by two chromatographic steps Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.053
-
pH 8.0, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
0.053
-
activity at final purification step Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + cob(I)alamin
-
Salmonella enterica subsp. enterica serovar Typhimurium triphosphate + coenzyme B12 adenosylcobalamin, B12-coenzyme or deoxyadenosyl-B12 ?

Subunits

Subunits Comment Organism
dimer 2 * 25000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
stable for several weeks with dithiothreitol Salmonella enterica subsp. enterica serovar Typhimurium
55
-
sharp decrease in activity with preincubation at 55°C or higher Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium