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Literature summary for 2.5.1.112 extracted from

  • Chu, H.M.; Ko, T.P.; Wang, A.H.
    Crystal structure and substrate specificity of plant adenylate isopentenyltransferase from Humulus lupulus: distinctive binding affinity for purine and pyrimidine nucleotides (2010), Nucleic Acids Res., 38, 1738-1748.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Humulus lupulus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with ATP, sitting drop vapor diffusion method, using 0.2 M ammonium tartrate and 20% (w/v) PEG 3350 Humulus lupulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0162
-
ATP in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C Humulus lupulus
0.0193
-
ADP in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C Humulus lupulus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + ADP Humulus lupulus
-
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
?
dimethylallyl diphosphate + ATP Humulus lupulus the enzme favors ATP as its ligand diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
?

Organism

Organism UniProt Comment Textmining
Humulus lupulus Q5GHF7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and Mono Q column chromatography Humulus lupulus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + ADP
-
Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
?
dimethylallyl diphosphate + ATP the enzme favors ATP as its ligand Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
?
dimethylallyl diphosphate + CTP
-
Humulus lupulus ?
-
?
dimethylallyl diphosphate + dATP the enzyme shows preference for dATP over ADP and 80% activity with dATP compared to ATP Humulus lupulus ?
-
?
dimethylallyl diphosphate + GTP
-
Humulus lupulus ?
-
?
dimethylallyl diphosphate + UTP
-
Humulus lupulus ?
-
?

Synonyms

Synonyms Comment Organism
adenylate isopentenyltransferase
-
Humulus lupulus
AIPT
-
Humulus lupulus

Cofactor

Cofactor Comment Organism Structure
ADP
-
Humulus lupulus
ATP
-
Humulus lupulus