Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Humulus lupulus |
Crystallization (Comment) | Organism |
---|---|
in complex with ATP, sitting drop vapor diffusion method, using 0.2 M ammonium tartrate and 20% (w/v) PEG 3350 | Humulus lupulus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0162 | - |
ATP | in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C | Humulus lupulus | |
0.0193 | - |
ADP | in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C | Humulus lupulus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + ADP | Humulus lupulus | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
dimethylallyl diphosphate + ATP | Humulus lupulus | the enzme favors ATP as its ligand | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humulus lupulus | Q5GHF7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Mono Q column chromatography | Humulus lupulus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + ADP | - |
Humulus lupulus | diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate | - |
? | |
dimethylallyl diphosphate + ATP | the enzme favors ATP as its ligand | Humulus lupulus | diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate | - |
? | |
dimethylallyl diphosphate + CTP | - |
Humulus lupulus | ? | - |
? | |
dimethylallyl diphosphate + dATP | the enzyme shows preference for dATP over ADP and 80% activity with dATP compared to ATP | Humulus lupulus | ? | - |
? | |
dimethylallyl diphosphate + GTP | - |
Humulus lupulus | ? | - |
? | |
dimethylallyl diphosphate + UTP | - |
Humulus lupulus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenylate isopentenyltransferase | - |
Humulus lupulus |
AIPT | - |
Humulus lupulus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Humulus lupulus | |
ATP | - |
Humulus lupulus |