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Literature summary for 2.5.1.10 extracted from

  • Ogura, K.; Nishino, T.; Seto, S.
    The purification of prenyltransferase and isopentenyl pyrophosphate isomerase of pumpkin fruit and their some properties (1968), J. Biochem., 64, 197-203.
    View publication on PubMed
Search for more literature for 2.5.1.10

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide 5 mM, 31% inhibition Pumpkin

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 geranyl diphosphate
-
 Pumpkin

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Pumpkin
Mn2+ can partially replace Mg2+, less effective Pumpkin

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + 2 isopentenyl diphosphate Pumpkin enzyme participates in isoprenoid biosynthesis in eukaryotes (E,E)-farnesyl diphosphate + 2 diphosphate
-
 ?
isopentenyl diphosphate + geranyl diphosphate Pumpkin enzyme participates in isoprenoid biosynthesis in eukaryotes diphosphate + farnesyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pumpkin
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G 200, hydroxylapatite Pumpkin

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Pumpkin
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 2 isopentenyl diphosphate activity is higher than with geranyl diphosphate Pumpkin (E,E)-farnesyl diphosphate + 2 diphosphate
-
 ?
dimethylallyl diphosphate + 2 isopentenyl diphosphate enzyme participates in isoprenoid biosynthesis in eukaryotes Pumpkin (E,E)-farnesyl diphosphate + 2 diphosphate
-
 ?
farnesyl diphosphate + isopentenyl diphosphate less than 5% of the activity with dimethylallyl diphosphate Pumpkin geranyl diphosphate + diphosphate
-
 ?
geranyl diphosphate + isopentenyl diphosphate
-
 Pumpkin diphosphate + farnesyl diphosphate
-
 ?
isopentenyl diphosphate + geranyl diphosphate enzyme participates in isoprenoid biosynthesis in eukaryotes Pumpkin diphosphate + farnesyl diphosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Pumpkin