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Literature summary for 2.4.3.4 extracted from

  • Fessner, W.D.; Mertsch, A.; He, N.; Yi, D.; Kickstein, M.
    An alpha2,3-sialyltransferase from Photobacterium phosphoreum with broad substrate scope Controlling hydrolytic activity by directed evolution (2020), Chem. Eur. J., 26, 11614-11624 .
    View publication on PubMed
Search for more literature for 2.4.3.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Photobacterium phosphoreum

Protein Variants

Protein Variants Comment Organism
A151D the mutant shows significantly improved properties as compared to the native enzyme. The mutant displays overall best performance in the synthesis of the GM3 trisaccharide (alpha2,3-Neu5Ac-Lac) from lactose in a one-pot, two-enzyme cascade Photobacterium phosphoreum
L387A the mutant shows significantly improved properties as compared to the native enzyme Photobacterium phosphoreum
S359T/S360T the mutant shows significantly improved properties as compared to the native enzyme Photobacterium phosphoreum

Organism

Organism UniProt Comment Textmining
Photobacterium phosphoreum A5LHX0
-
-
Photobacterium phosphoreum JT-ISH-467 A5LHX0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity column chromatography Photobacterium phosphoreum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetyl-beta-neuraminate + lactose
-
 Photobacterium phosphoreum CMP + alpha2,3-SiaLac + Neu5Ac
-
 ?
CMP-N-acetyl-beta-neuraminate + lactose
-
 Photobacterium phosphoreum JT-ISH-467 CMP + alpha2,3-SiaLac + Neu5Ac
-
 ?

Synonyms

Synonyms Comment Organism
2,3SiaT
-
 Photobacterium phosphoreum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47.1
-
 melting temperature Photobacterium phosphoreum