Literature summary for 2.4.2.8 extracted from

Kanagawa, M.; Baba, S.; Ebihara, A.; Shinkai, A.; Hirotsu, K.; Mega, R.; Kim, K.; Kuramitsu, S.; Sampei, G.; Kawai, G.
Structures of hypoxanthine-guanine phosphoribosyltransferase (TTHA0220) from Thermus thermophilus HB8 (2010), Acta Crystallogr. Sect. F, 66, 893-898.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
structure in the unliganded form, in complex with IMP and in complex with GMP, to 2.1, 1.9 and 2.2 A resolution, respectively. The overall fold of the IMP complex is similar to that of the unliganded form, but the main-chain and side-chain atoms of the active site move to accommodate IMP. The overall folds of the IMP and GMP complexes are almost identical to each other. Enzyme belongs to group I	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0039	-	hypoxanthine	pH 7.9, 25°C	Thermus thermophilus
0.0074	-	guanine	pH 7.9, 25°C	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q5SLS3	-	-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579	Q5SLS3	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate	-	Thermus thermophilus	GMP + diphosphate	-	?
guanine + 5-phospho-alpha-D-ribose 1-diphosphate	-	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	GMP + diphosphate	-	?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate	-	Thermus thermophilus	IMP + diphosphate	-	?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate	-	Thermus thermophilus HB8 / ATCC 27634 / DSM 579	IMP + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
HGPRTase	-	Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
9.1	-	hypoxanthine	pH 7.9, 25°C	Thermus thermophilus
20	-	guanine	pH 7.9, 25°C	Thermus thermophilus

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Release 2023.1 (January 2023)