Application | Comment | Organism |
---|---|---|
medicine | potential target for antiparasitic chemotherapy | Homo sapiens |
medicine | potential target for antiparasitic chemotherapy | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
overexpression in an enzyme-deficient Escherichia coli strain | Plasmodium falciparum |
overexpression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C105A | prepared via splicing by overlap extension, reduced oxidation ofthe enzyme during storage | Homo sapiens |
C205A | prepared via splicing by overlap extension, reduced oxidation ofthe enzyme during storage | Homo sapiens |
C22A | prepared via splicing by overlap extension, reduced oxidation ofthe enzyme during storage | Homo sapiens |
General Stability | Organism |
---|---|
DTT and hypoxanthine stabilize | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | complete inhibition at 3 mM after 3 min at 0°C | Homo sapiens | |
Hg2+ | complete inhibition at 3 mM after 3 min at 0°C | Plasmodium falciparum | |
KCl | no inhibition | Homo sapiens | |
KCl | inactivation | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
hypoxanthine | recombinant enzyme, with other purine | Plasmodium falciparum | |
0.0014 | - |
guanine | recombinant enzyme, with other purine | Plasmodium falciparum | |
0.0019 | - |
guanine | recombinant enzyme, with other purine | Homo sapiens | |
0.0031 | - |
hypoxanthine | recombinant enzyme, with other purine | Homo sapiens | |
0.0117 | - |
allopurinol | recombinant enzyme | Plasmodium falciparum | |
0.06 | - |
hypoxanthine | recombinant enzyme, with 5-phosphoribosyl 1-diphosphate | Plasmodium falciparum | |
0.126 | - |
hypoxanthine | recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5 | Homo sapiens | |
0.134 | - |
guanine | recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5 | Homo sapiens | |
0.135 | - |
allopurinol | recombinant enzyme | Homo sapiens | |
0.17 | - |
xanthine | recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5 | Plasmodium falciparum | |
0.362 | - |
guanine | recombinant enzyme, with 5-phosphoribosyl 1-diphosphate | Plasmodium falciparum | |
0.42 | - |
xanthine | recombinant enzyme, pH 8.5 | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Homo sapiens |
recombinant from Escherichia coli , large scale | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.27 | - |
purified recombinant enzyme, substrate allopurinol, pH 8.0 | Homo sapiens |
1.4 | - |
purified recombinant enzyme, substrate hypoxanthine, pH 8.0 | Plasmodium falciparum |
3 | - |
purified recombinant enzyme, substrates allopurinol and xanthine, pH 8.0 | Plasmodium falciparum |
5.4 | - |
purified recombinant enzyme, substrate guanine, pH 8.0 | Plasmodium falciparum |
27 | - |
purified recombinant enzyme, substrate hypoxanthine, pH 8.0 | Homo sapiens |
46 | - |
purified recombinant enzyme, substrate guanine, pH 8.0 | Homo sapiens |
Storage Stability | Organism |
---|---|
0°C, 10 mM phosphate, pH 7.1, 1 mM DTT, 10 mM Mg2+, 2 h, no loss of activity | Homo sapiens |
4°C, freshly purified enzyme, 10 mM phosphate, pH 6.8, 10 mM DTT, 1 mM 5-phosphoribosyl 1-diphosphate, rapid loss of 90% activity within 48 h | Plasmodium falciparum |
5°C, purified enzyme, 10 mM phosphate, pH 6.8, 1 mM DTT, 0.2 mM 5-phosphoribosyl 1-diphosphate, 0.06 hypoxanthine, loss of 7% activity after 4-8 weeks | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | allopurinol ribonucleoside 5'-monophosphate + diphosphate | - |
? | |
allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate | low activity | Homo sapiens | allopurinol ribonucleoside 5'-monophosphate + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | GMP + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | XMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | XMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24352-24353, recombinant mutant enzymes, mass spectroscopy and DNA sequence determination | Homo sapiens |
dimer | - |
Homo sapiens |
dimer | 2 * 26229-26232, in presence of KCl, SDS-PAGE, mass spectroscopy and DNA sequence determination | Plasmodium falciparum |
tetramer | - |
Homo sapiens |
tetramer | 4 * 26229-26232, in absence of KCl, SDS-PAGE, mass spectroscopy and DNA sequence determination | Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Plasmodium falciparum |