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Literature summary for 2.4.2.8 extracted from
- Hypoxanthine phosphoribosyltransferase from Chinese hamster brain and human erythrocytes (1978), Methods Enzymol., 51, 543-549.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00052 | - |
hypoxanthine | - |
Cricetulus griseus |  |
| 0.0011 | - |
guanine | - |
Cricetulus griseus | |
| 0.0011 | - |
guanine | - |
Homo sapiens | |
| 0.0053 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Cricetulus griseus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
3 * 25000, SDS-PAGE | Cricetulus griseus |
| 26000 | - |
3 * 26000, SDS-PAGE | Homo sapiens |
| 78000 | 85000 | gel filtration | Cricetulus griseus |
| 80000 | 85000 | gel filtration, acrylamide gel electrophoresis | Cricetulus griseus |
| 81000 | 83000 | sedimentation equilibrium centrifugation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cricetulus griseus | - |
at least 3 charge variant isoforms | - |
| Homo sapiens | - |
3 charge variant forms | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cricetulus griseus |
| from erythrocytes and from brain | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Cricetulus griseus | - |
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 9 | - |
purified enzyme, brain | Cricetulus griseus |
| 17.5 | - |
purified enzyme, erythrocytes | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Cricetulus griseus | GMP + diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Cricetulus griseus | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 26000, SDS-PAGE | Homo sapiens |
| trimer | 3 * 25000, SDS-PAGE | Cricetulus griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cricetulus griseus |
| 37 | - |
assay at | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
if first incubated in 1 mM 5-phospho-alpha-D-ribose 1-diphosphate, remarkably stable | Cricetulus griseus |
| 85 | - |
if first incubated in 1 mM 5-phospho-alpha-D-ribose 1-diphosphate, remarkably stable | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
3 charge variant forms with pIs of 5.6, 5.7 and 5.9 | Homo sapiens |
| additional information | - |
3 charge variant forms with pIs of 6.2, 6.4 and 6.6 | Cricetulus griseus |
| 7.8 | - |
assay at | Homo sapiens |
| 10 | - |
- |
Cricetulus griseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 11 | - |
Cricetulus griseus |
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