Literature summary for 2.4.2.6 extracted from

Fernandez-Lucas, J.; Fresco-Taboada, A.; de la Mata, I.; Arroyo, M.
One-step enzymatic synthesis of nucleosides from low water-soluble purine bases in non-conventional media (2012), Biores. Technol., 115, 63-69.

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Application

Application	Comment	Organism
synthesis	immobilization of 2'-deoxyribosyltransferase from Lactobacillus reuteri on Sepabeads. Immobilized enzyme activity is enhanced 1.21.4fold at 20% of methanol, ethanol, 2-propanol, diethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increased activity is also obtained in presence of 20% acetonitrile. Immobilized enzyme is successfully used in the synthesis of 2'-deoxyxanthosine and 2'-deoxyguanosine using 2'-deoxyuridine as sugar donor and the corresponding poor water-soluble base in the presence of 30% of methanol, ethanol, 2-propanol, ethylene glycol, acetonitrile, and DMSO, giving high nucleoside yields at 4 h	Limosilactobacillus reuteri

Organic Solvent Stability

Organic Solvent	Comment	Organism
2-propanol	enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile	Limosilactobacillus reuteri
Acetone	enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile	Limosilactobacillus reuteri
acetonitrile	enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile	Limosilactobacillus reuteri
Ethanol	enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile	Limosilactobacillus reuteri
Ethylene glycol	enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile	Limosilactobacillus reuteri
Methanol	enzyme immobilized on Sepabeads shows 1.2-1.4fold enhanced activity at 20% of methanol, ethanol, 2-propanol, ethylene glycol, and acetone, and at 10% and 30% acetonitrile. Highest increase in activity is obtained in presence of 20% acetonitrile	Limosilactobacillus reuteri

Organism

Organism	UniProt	Comment	Textmining
Limosilactobacillus reuteri	A5VK58	-	-

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Release 2023.1 (January 2023)