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Literature summary for 2.4.2.6 extracted from
- Trans-N-deoxyribosylase from Lactobacillus helveticus. Crystallization and properties (1971), Eur. J. Biochem., 23, 387-395.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| microdiffusion by means of equilibrium dialysis is used to crystallize the enzyme | Lactobacillus helveticus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 82000 | - |
gel filtration | Lactobacillus helveticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus helveticus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus helveticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 23.1 | - |
- |
Lactobacillus helveticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -50°C, stable for several months either in the lyophilized form or disolved in freshly distilled water | Lactobacillus helveticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxycytidine + adenine | - |
Lactobacillus helveticus | cytosine + deoxyadenosine | - |
r |  |
| deoxycytidine + guanine | - |
Lactobacillus helveticus | cytosine + deoxyguanosine | - |
r | |
| deoxycytidine + hypoxanthine | - |
Lactobacillus helveticus | cytosine + deoxyinosine | - |
r | |
| deoxyguanosine + adenine | - |
Lactobacillus helveticus | guanine + deoxyadenosine | - |
r | |
| deoxyinosine + adenine | - |
Lactobacillus helveticus | hypoxanthine + deoxyadenosine | - |
r | |
| deoxyuridine + adenine | - |
Lactobacillus helveticus | deoxyadenosine + uracil | - |
? | |
| deoxyuridine + guanine | - |
Lactobacillus helveticus | deoxyguanosine + uracil | - |
? | |
| deoxyuridine + hypoxanthine | - |
Lactobacillus helveticus | deoxyinosine + uracil | - |
? | |
| thymidine + adenine | - |
Lactobacillus helveticus | thymine + 2'-deoxyadenosine | - |
? | |
| thymidine + cytosine | - |
Lactobacillus helveticus | thymine + 2'-deoxycytidine | - |
? | |
| thymidine + guanine | - |
Lactobacillus helveticus | 2'-deoxyguanosine + thymine | - |
? | |
| thymidine + hypoxanthine | - |
Lactobacillus helveticus | deoxyinosine + thymine | - |
? | |
| thymidine + uracil | - |
Lactobacillus helveticus | deoxyuridine + thymine | - |
? | |
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