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Literature summary for 2.4.2.57 extracted from

  • Aono, R.; Sato, T.; Yano, A.; Yoshida, S.; Nishitani, Y.; Miki, K.; Imanaka, T.; Atomi, H.
    Enzymatic characterization of AMP phosphorylase and ribose-1,5-bisphosphate isomerase functioning in an archaeal AMP metabolic pathway (2012), J. Bacteriol., 194, 6847-6855.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.57

Activating Compound

Activating Compound Comment Organism Structure
cAMP the activity with CMP and UMP requires activation by cAMP Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 AMP the kinetic parameters of the enzyme toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
2.8
-
 phosphate pH 7.5, 85°C Thermococcus kodakarensis
2.8
-
 phosphate pH 7.5, 85°C, cosubstrate: AMP Thermococcus kodakarensis
4.4
-
 UMP pH 7.5, 85°C Thermococcus kodakarensis
6.2
-
 CMP pH 7.5, 85°C Thermococcus kodakarensis
18.5
-
 GMP pH 7.5, 85°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
AMP + phosphate Thermococcus kodakarensis the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?
AMP + phosphate Thermococcus kodakarensis the enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JCX3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermococcus kodakarensis

Reaction

Reaction Comment Organism Reaction ID
AMP + phosphate = adenine + alpha-D-ribose 1,5-bisphosphate (1) Thermococcus kodakarensis
a
CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate (2) Thermococcus kodakarensis
a
UMP + phosphate = uracil + alpha-D-ribose 1,5-bisphosphate (3) Thermococcus kodakarensis
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
 substrate: GMP, pH 7.5, 85°C Thermococcus kodakarensis
10.8
-
 substrate: UMP, pH 7.5, 85°C Thermococcus kodakarensis
15.9
-
 substrate: AMP, pH 7.5, 85°C Thermococcus kodakarensis
37.5
-
 substrate: CMP, pH 7.5, 85°C Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + phosphate
-
 Thermococcus kodakarensis adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?
AMP + phosphate the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes Thermococcus kodakarensis adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?
AMP + phosphate the enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes Thermococcus kodakarensis adenine + alpha-D-ribose 1,5-bisphosphate
-
 ?
CMP + phosphate
-
 Thermococcus kodakarensis cytosine + alpha-D-ribose 1,5-bisphosphate
-
 ?
GMP + phosphate
-
 Thermococcus kodakarensis guanine + alpha-D-ribose 1,5-bisphosphate
-
 ?
additional information the use of dAMP, dGMP, or dUMP results in the generation of only trace amounts of their corresponding nucleobases, adenine, guanine, or uracil, respectivelyno activity with: TMP, ADP, ATP, dCMP, dAMP, dGMP, dUMP Thermococcus kodakarensis ?
-
 ?
UMP + phosphate
-
 Thermococcus kodakarensis uracil + alpha-D-ribose 1,5-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
AMPpase
-
 Thermococcus kodakarensis
deoA
-
 Thermococcus kodakarensis
nucleoside monophosphate phosphorylase
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 assay at Thermococcus kodakarensis
85
-
 assay Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
additional information
-
 AMP the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
2.7
-
 GMP pH 7.5, 85°C Thermococcus kodakarensis
10.5
-
 UMP pH 7.5, 85°C Thermococcus kodakarensis
15
-
 phosphate pH 7.5, 85°C Thermococcus kodakarensis
15
-
 phosphate pH 7.5, 85°C, cosubstrate: AMP Thermococcus kodakarensis
39.1
-
 CMP pH 7.5, 85°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 additional information the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
additional information
-
 AMP the kinetic parameters of the AMPpase reaction toward AMP are not determined, because kinetic equations that fit the data well can not be identified Thermococcus kodakarensis
0.1
-
 GMP pH 7.5, 85°C Thermococcus kodakarensis
2.4
-
 UMP pH 7.5, 85°C Thermococcus kodakarensis
5.4
-
 phosphate pH 7.5, 85°C, cosubstrate: AMP Thermococcus kodakarensis
6.3
-
 CMP pH 7.5, 85°C Thermococcus kodakarensis