Cloned (Comment) | Organism |
---|---|
the 6*His-tagged ArnT gene-encoded plasmids are transformed into Escherichia coli NovaBlue (Novagen) cells for protein expression. The gene is knocked out of the chromosomal DNA of BL21 (BL21(DE3)DELTAArnT), leaving the plasmid-encoded ArnT to be the only ArnT expressed in the system | Salmonella enterica subsp. enterica serovar Typhimurium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
x * 59000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,poly-cis-undecaprenyl phosphate + lipid IVA | Salmonella enterica subsp. enterica serovar Typhimurium | - |
lipid IIA + di-trans,poly-cis-undecaprenyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | O52327 | - |
- |
Purification (Comment) | Organism |
---|---|
purifiation of 6*His-tagged ArnT. HisLink nickel affinity chromatography coupled with anion exchange chromatography results in the best overall purifiation strategy for dodecylmaltoside-solubilized ArnT | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,poly-cis-undecaprenyl phosphate + lipid IVA | - |
Salmonella enterica subsp. enterica serovar Typhimurium | lipid IIA + di-trans,poly-cis-undecaprenyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 59000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
4-amino-4-deoxy-L-arabinose transferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
arnT | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
L-Ara4N transferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |