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Literature summary for 2.4.2.43 extracted from

  • Bretscher, L.E.; Morrell, M.T.; Funk, A.L.; Klug, C.S.
    Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium (2006), Protein Expr. Purif., 46, 33-39.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the 6*His-tagged ArnT gene-encoded plasmids are transformed into Escherichia coli NovaBlue (Novagen) cells for protein expression. The gene is knocked out of the chromosomal DNA of BL21 (BL21(DE3)DELTAArnT), leaving the plasmid-encoded ArnT to be the only ArnT expressed in the system Salmonella enterica subsp. enterica serovar Typhimurium

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane
-
Salmonella enterica subsp. enterica serovar Typhimurium
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
x * 59000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,poly-cis-undecaprenyl phosphate + lipid IVA Salmonella enterica subsp. enterica serovar Typhimurium
-
lipid IIA + di-trans,poly-cis-undecaprenyl phosphate
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium O52327
-
-

Purification (Commentary)

Purification (Comment) Organism
purifiation of 6*His-tagged ArnT. HisLink nickel affinity chromatography coupled with anion exchange chromatography results in the best overall purifiation strategy for dodecylmaltoside-solubilized ArnT Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-amino-4-deoxy-alpha-L-arabinopyranosyl di-trans,poly-cis-undecaprenyl phosphate + lipid IVA
-
Salmonella enterica subsp. enterica serovar Typhimurium lipid IIA + di-trans,poly-cis-undecaprenyl phosphate
-
?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
4-amino-4-deoxy-L-arabinose transferase
-
Salmonella enterica subsp. enterica serovar Typhimurium
arnT
-
Salmonella enterica subsp. enterica serovar Typhimurium
L-Ara4N transferase
-
Salmonella enterica subsp. enterica serovar Typhimurium