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Literature summary for 2.4.2.4 extracted from

  • Schwartz, M.
    Thymidine phosphorylase from Escherichia coli (1978), Methods Enzymol., 51, 442-445.
    View publication on PubMed

General Stability

General Stability Organism
stability depends on protein concentration Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
ribose 1-phosphate competitive Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
thymidine
-
Escherichia coli
0.89
-
phosphate
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate ordered sequential reaction mechanism, phosphate is the first substrate to bind to and deoxyribose the last product to dissociate from the enzyme Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
122
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris-HCl, pH 7.3, stable for several months Escherichia coli
4°C, gradual loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: deoxycytidine Escherichia coli ?
-
?
additional information specific for deoxyribonucleosides Escherichia coli ?
-
?
additional information purine deoxyribonucleosides and ribonucleosides are not cleaved Escherichia coli ?
-
?
thymidine + arsenate
-
Escherichia coli ?
-
?
thymidine + phosphate
-
Escherichia coli thymine + 2-deoxy-D-ribose 1-phosphate
-
?

Subunits

Subunits Comment Organism
dimer two identical subunits, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
Escherichia coli
7.1
-
assay at Escherichia coli