Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Spodoptera frugiperda SF21 cells using the baculovirus infection system | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several N-terminal deletion mutants, the first 54 residues can be deleted without affecting the catalytic activity of the enzyme, removal of an additional five amino acids lead to the formation of an inactive protein | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 59% inhibition at 10 mM | Arabidopsis thaliana | |
EDTA | 20% inhibition at 10 mM | Arabidopsis thaliana | |
Ni2+ | 99% inhibition at 10 mM | Arabidopsis thaliana | |
UDP | competitive | Arabidopsis thaliana | |
UDP-glucose | - |
Arabidopsis thaliana | |
Zn2+ | 95% inhibition at 10 mM | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
UDP-xylose | pH 7.0, 37°C, recombinant mutant DELTA39 XylT | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Arabidopsis thaliana | 5794 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirements for metal ions | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | the enzyme is involved in the biosynthesis of glycoprotein-bound N-glycans in plants and acts at multiple stages of the plant N-glycosylation pathway, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | three potential N-glycosylation sites of the wild-type enzyme as well as of the recombinant enzyme are occupied by N-linked oligosaccharides, N-glycosylation is not essential for proper folding of XylT | Arabidopsis thaliana |
proteolytic modification | proteolytic processing of recombinant XylT occurs between amino acids Arg178 and Arg179, a cleavage sequence compatible with the enzymatic properties of the baculoviral cysteine proteinase | Arabidopsis thaliana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-xylose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = UDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-[beta-D-Xyl-(1->2)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] | the catalytic domain of XylT encompasses residues Lys57-Cys534 | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Xylbeta1-2GnGn)-GP + UDP-xylose | i.e. GnGnX | Arabidopsis thaliana | ? | - |
? | |
GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-O-octyl + UDP-xylose | i.e. GnGn-octyl | Arabidopsis thaliana | ? | - |
? | |
additional information | the enzyme is involved in the biosynthesis of glycoprotein-bound N-glycans in plants and acts at multiple stages of the plant N-glycosylation pathway, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | the enzyme is specific for UDP-xylose, no activity with Man5-GP and MM-octyl, acceptor substrate specificity, overview | Arabidopsis thaliana | ? | - |
? | |
Xylbeta1-2[Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)]Manbeta1-O-octyl + UDP-xylose | i.e. MGnX-octyl | Arabidopsis thaliana | ? | - |
? | |
Xylbeta1-2[Manalpha1-6(Manalpha1-3)]Manbeta1-O-octyl + UDP-xylose | i.e. MMX-octyl | Arabidopsis thaliana | ? | - |
? | |
[GlcNAcbeta1-2Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-3)GlcNAc]-GP + UDP-xylose | i.e. GnGnF | Arabidopsis thaliana | ? | - |
? | |
[Manalpha1-6(Manalpha1-3)Manalpha1-6(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlcNAc]-GP + UDP-xylose | i.e. Man5Gn | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | primary sequence and hydrophobicity analysis of recombinant XylT | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
beta1,2-xylosyltransferase | - |
Arabidopsis thaliana |
XylT | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
the purified enzyme is stable for prolonged periods at neutral pH and at temperatures of up to 30°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | recombinant mutant DELTA39 XylT | Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the purified enzyme is stable for prolonged periods at neutral pH and at temperatures of up to 30°C | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
UDP | pH 7.0, 37°C, recombinant mutant DELTA39 XylT | Arabidopsis thaliana | |
4.9 | - |
UDP-glucose | pH 7.0, 37°C, recombinant mutant DELTA39 XylT | Arabidopsis thaliana |