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Literature summary for 2.4.2.38 extracted from
- A genetic and structural analysis of the N-glycosylation capabilities of rice and other monocotyledons (2004), Plant Mol. Biol., 55, 631-644.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, gene is located on chromosome 8, functional expression of the enzyme in Pichia pastoris as soluble protein, secretion to the cell culture medium | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Oryza sativa |  |
| Cu2+ | - |
Oryza sativa | |
| EDTA | - |
Oryza sativa | |
| Zn2+ | - |
Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc | recombinant soluble enzyme, pH 6.0, 16°C | Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | - |
Oryza sativa | 5794 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates, enzyme requires divalent cations for full activity | Oryza sativa | |
| Fe2+ | activates, enzyme requires divalent cations for full activity | Oryza sativa | |
| Mg2+ | activates, enzyme requires divalent cations for full activity | Oryza sativa | |
| Mn2+ | activates, enzyme requires divalent cations for full activity | Oryza sativa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Oryza sativa | enzyme is involved in N-glycan biosynthesis, quantification and structure analysis of N-glycans in rice leaves | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | Q6ZFH6 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Oryza sativa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified recombinant enzyme, 3 weeks stable without loss of activity | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme is involved in N-glycan biosynthesis, quantification and structure analysis of N-glycans in rice leaves | Oryza sativa | ? | - |
? | |
| UDP-xylose + dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc | i.e. dansylated GnGn-peptide | Oryza sativa | UDP + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta1,2-xylosyltransferase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 16 | - |
- |
Oryza sativa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
low activity at 30°C | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
- |
Oryza sativa |
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