Literature summary for 2.4.2.37 extracted from

Moure, V.R.; Danyal, K.; Yang, Z.Y.; Wendroth, S.; Mueller-Santos, M.; Pedrosa, F.O.; Scarduelli, M.; Gerhardt, E.C.; Huergo, L.F.; Souza, E.M.; Seefeldt, L.C.
The nitrogenase regulatory enzyme dinitrogenase reductase ADP-ribosyltransferase (DraT) is activated by direct interaction with the signal transduction protein GlnB (2013), J. Bacteriol., 195, 279-286.

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Activating Compound

Activating Compound	Comment	Organism	Structure
protein GlnB	GlnB, a nitrogen-signaling PII protein, is necessary for DraT activity in the presence of Mg-ADP	Azospirillum brasilense
protein GlnZ	GlnZ, a nitrogen-signaling PII protein, activates DraT activity	Azospirillum brasilense

Inhibitors

Inhibitors	Comment	Organism	Structure
2-oxoglutarate	-	Azospirillum brasilense

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.166	-	NAD+	pH 7.5, 30°C, Fe protein as the acceptor of ADP-ribose, in the presence of MgADP-	Azospirillum brasilense

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	MgADP- required. DraT-GlnB is ineffective in the presence of MgATP2-	Azospirillum brasilense

Organism

Organism	UniProt	Comment	Textmining
Azospirillum brasilense	Q9JP48	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + dinitrogen reductase	Fe protein as the acceptor of ADP-ribose	Azospirillum brasilense	nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]	-	?

Synonyms

Synonyms	Comment	Organism
dinitrogenase reductase ADP-ribosyltransferase	-	Azospirillum brasilense
DRAT	-	Azospirillum brasilense

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Azospirillum brasilense

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.501	-	NAD+	pH 7.5, 30°C, Fe protein as the acceptor of ADP-ribose, in the presence of MgADP-	Azospirillum brasilense

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Azospirillum brasilense

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
additional information	-	additional information	the DraT-GlnB complex is at least 18fold more efficient than DraT purified from Rhodospirillum rubrum	Azospirillum brasilense
3.018	-	NAD+	pH 7.5, 30°C, Fe protein as the acceptor of ADP-ribose, in the presence of MgADP-	Azospirillum brasilense

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Release 2023.1 (January 2023)