Literature summary for 2.4.2.36 extracted from

Jang, H.; Kim, H.S.; Kim, J.A.; Seo, J.H.; Carbis, R.
Improved purification process for cholera toxin and its application to the quantification of residual toxin in cholera vaccines (2009), J. Microbiol. Biotechnol., 19, 108-112.

Search for more literature for 2.4.2.36

Application

Application	Comment	Organism
diagnostics	toxin purification and recognition to enable cost effective toxin free cholera vaccine production, detection minimum 1 ng/ml	Vibrio cholerae serotype O1

Organism

Organism	UniProt	Comment	Textmining
Vibrio cholerae serotype O1	-	hyper-producer of cholera toxin	-
Vibrio cholerae serotype O1 569B	-	hyper-producer of cholera toxin	-

Purification (Commentary)

Purification (Comment)	Organism
0.2 microm crossflow microfiltration, concentrated and diafiltered against 10 mM phosphate buffer, pH 7.6, with 30 kDa crossflow ultrafiltration, toxin bound on a S 5/50 GL cation exchange column and eluted with a salt gradient of 10 mM potassium phosphate buffer, pH 7.0 with 1 M NaCl	Vibrio cholerae serotype O1

Synonyms

Synonyms	Comment	Organism
cholera toxin	-	Vibrio cholerae serotype O1

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)