Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-((3,3-diethylguanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 24% inhibition | Vibrio cholerae serotype O1 | |
3-((3,3-diisopropylguanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 94% inhibition | Vibrio cholerae serotype O1 | |
3-((3-(1-benzylpiperidin-4-yl)guanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 96% inhibition | Vibrio cholerae serotype O1 | |
3-((3-(1-benzylpyrrolidin-3-yl)guanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 68% inhibition | Vibrio cholerae serotype O1 | |
3-((3-(2-(ethyl(m-tolyl)amino)ethyl)guanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 76% inhibition | Vibrio cholerae serotype O1 | |
3-((3-(2-morpholinoethyl)guanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 57% inhibition | Vibrio cholerae serotype O1 | |
3-((3-(4-bromobenzyl)guanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 98% inhibition | Vibrio cholerae serotype O1 | |
3-((3-(4-methoxybenzyl)guanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 97% inhibition, not sufficient material for IC50 and Ki estimation | Vibrio cholerae serotype O1 | |
3-((3-benzylguanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 93% inhibition | Vibrio cholerae serotype O1 | |
3-((3-phenethylguanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 93% inhibition | Vibrio cholerae serotype O1 | |
3-((3-phenylguanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 57% inhibition | Vibrio cholerae serotype O1 | |
3-((3-tert-butylguanidino)methyl)benzamide | 1 mM, 37°C, pH 7, 39% inhibition | Vibrio cholerae serotype O1 | |
3-(3,3-diisopropylguanidino)benzamide | 1 mM, 37°C, pH 7, 15% inhibition | Vibrio cholerae serotype O1 | |
3-(3-(1-benzylpiperidin-4-yl)guanidino)benzamide | 1 mM, 37°C, pH 7, 58% inhibition | Vibrio cholerae serotype O1 | |
3-(3-(4-bromobenzyl)guanidino)benzamide | 1 mM, 37°C, pH 7, 22% inhibition | Vibrio cholerae serotype O1 | |
3-(3-benzylguanidino)benzamide | 1 mM, 37°C, pH 7, 10% inhibition | Vibrio cholerae serotype O1 | |
3-(3-phenylguanidino)benzamide | 1 mM, 37°C, pH 7, 12% inhibition | Vibrio cholerae serotype O1 | |
3-(3-tert-butylguanidino)benzamide | 1 mM, 37°C, pH 7, 77% inhibition | Vibrio cholerae serotype O1 | |
ethyl 4-(3-(3-carbamoylbenzyl)guanidino)piperidine-1-carboxylate | 1 mM, 37°C, pH 7, 34% inhibition | Vibrio cholerae serotype O1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
diethylamino(benzylidine-amino)guanidine | - |
Vibrio cholerae serotype O1 | |
14 | - |
NAD+ | - |
Vibrio cholerae serotype O1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + G-protein | Vibrio cholerae serotype O1 | NAD-dependent ADP-ribosylation of Arg201 of G-protein alpha-subunit, this locks the G-protein in its active state producing cAMP | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae serotype O1 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Vibrio cholerae serotype O1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethylamino(benzylidine-amino)guanidine + NAD+ | DEABAG | Vibrio cholerae serotype O1 | ? | - |
? | |
NAD+ + G-protein | NAD-dependent ADP-ribosylation of Arg201 of G-protein alpha-subunit, this locks the G-protein in its active state producing cAMP | Vibrio cholerae serotype O1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | catalytically active A subunit and five identical B subunits for receptor binding | Vibrio cholerae serotype O1 |
Synonyms | Comment | Organism |
---|---|---|
cholera toxin | - |
Vibrio cholerae serotype O1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
3-((3-(4-bromobenzyl)guanidino)methyl)benzamide | 37°C, pH 7, 1400fold more potent than NAD+ and 400fold more potent than diethylamino(benzylidine-amino)guanidine | Vibrio cholerae serotype O1 | |
0.013 | - |
3-((3-(1-benzylpiperidin-4-yl)guanidino)methyl)benzamide | 37°C, pH 7 | Vibrio cholerae serotype O1 | |
0.03 | - |
3-((3,3-diisopropylguanidino)methyl)benzamide | 37°C, pH 7 | Vibrio cholerae serotype O1 | |
0.05 | - |
3-((3-benzylguanidino)methyl)benzamide | 37°C, pH 7 | Vibrio cholerae serotype O1 | |
0.071 | - |
3-((3-phenethylguanidino)methyl)benzamide | 37°C, pH 7 | Vibrio cholerae serotype O1 | |
0.09 | - |
3-(3,3-diisopropylguanidino)benzamide | 37°C, pH 7 | Vibrio cholerae serotype O1 | |
0.5 | - |
3-((3-tert-butylguanidino)methyl)benzamide | 37°C, pH 7 | Vibrio cholerae serotype O1 | |
0.5 | - |
3-(3-phenylguanidino)benzamide | 37°C, pH 7 | Vibrio cholerae serotype O1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.031 | - |
37°C, pH 7 | Vibrio cholerae serotype O1 | 3-((3-(4-bromobenzyl)guanidino)methyl)benzamide | |
0.049 | - |
37°C, pH 7 | Vibrio cholerae serotype O1 | 3-((3-(1-benzylpiperidin-4-yl)guanidino)methyl)benzamide | |
0.115 | - |
37°C, pH 7 | Vibrio cholerae serotype O1 | 3-((3,3-diisopropylguanidino)methyl)benzamide | |
0.192 | - |
37°C, pH 7 | Vibrio cholerae serotype O1 | 3-((3-benzylguanidino)methyl)benzamide | |
0.272 | - |
37°C, pH 7 | Vibrio cholerae serotype O1 | 3-((3-phenethylguanidino)methyl)benzamide | |
0.35 | - |
37°C, pH 7 | Vibrio cholerae serotype O1 | 3-(3,3-diisopropylguanidino)benzamide | |
2 | - |
37°C, pH 7 | Vibrio cholerae serotype O1 | 3-((3-tert-butylguanidino)methyl)benzamide | |
2 | - |
37°C, pH 7 | Vibrio cholerae serotype O1 | 3-(3-phenylguanidino)benzamide |