Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
C11S | the mutation results in an auto-ADP-ribosylation comparable to the wild type enzyme | Neisseria meningitidis |
C128S | the mutant does not have a stable Fe-S cluster and reduced auto-ADP-ribosylation activity | Neisseria meningitidis |
C130S | the mutation results in an auto-ADP-ribosylation comparable to the wild type enzyme | Neisseria meningitidis |
C67S | the mutant does not have a stable Fe-S cluster and reduced auto-ADP-ribosylation activity | Neisseria meningitidis |
E109D | the mutation results in an auto-ADP-ribosylation comparable to the wild type enzyme | Neisseria meningitidis |
E111D | the mutation results in an auto-ADP-ribosylation comparable to the wild type enzyme | Neisseria meningitidis |
E120D | the mutant has no auto-ADP-ribosylation ability | Neisseria meningitidis |
R7K | the mutant has no auto-ADP-ribosylation ability | Neisseria meningitidis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
novobiocin | - |
Neisseria meningitidis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | required for activity | Neisseria meningitidis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18000, SDS-PAGE | Neisseria meningitidis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Neisseria meningitidis | the enzyme can perform auto-ADP-ribosylation. The auto-ADP-ribosylation site occurs preferentially on the R7 residue | ? | - |
? | |
NAD+ + polyarginine | Neisseria meningitidis | - |
nicotinamide + Nomega-(ADP-D-ribosyl)-polyarginine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | Q9JZ10 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Neisseria meningitidis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme can perform auto-ADP-ribosylation. The auto-ADP-ribosylation site occurs preferentially on the R7 residue | Neisseria meningitidis | ? | - |
? | |
additional information | the enzyme also exhibits NADase activity which produces free ADP-ribose that can covalently bind to lysine | Neisseria meningitidis | ? | - |
? | |
NAD+ + polyarginine | - |
Neisseria meningitidis | nicotinamide + Nomega-(ADP-D-ribosyl)-polyarginine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18000, SDS-PAGE | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
ADP-ribosyltransferase | - |
Neisseria meningitidis |
NarE | - |
Neisseria meningitidis |