Literature summary for 2.4.2.30 extracted from

Krska, D.; Ravulapalli, R.; Fieldhouse, R.J.; Lugo, M.R.; Merrill, A.R.
C3larvin toxin, an ADP-ribosyltransferase from Paenibacillus larvae (2015), J. Biol. Chem., 290, 1639-1653.

Search for more literature for 2.4.2.30

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Paenibacillus larvae subsp. larvae

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 28% (w/v) PEG 4000, 200 mM sodium acetate, and 100 mM Tris, pH 8.5	Paenibacillus larvae subsp. larvae

Inhibitors

Inhibitors	Comment	Organism	Structure
N-[[1-(1H-pyrazolo[3,4-d]pyrimidin-4-yl)piperidin-3-yl]methyl]methanesulfonamide	-	Paenibacillus larvae subsp. larvae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.017	-	(ADP-D-ribosyl)n-RhoA protein	at pH 7.4 and 37°C	Paenibacillus larvae subsp. larvae
0.034	-	NAD+	at pH 7.4 and 37°C	Paenibacillus larvae subsp. larvae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
22000	-	x * 22000, calculated from amino acid sequence	Paenibacillus larvae subsp. larvae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NAD+ + (ADP-D-ribosyl)n-RhoA protein	Paenibacillus larvae subsp. larvae	-	nicotinamide + (ADP-D-ribosyl)n+1-RhoA protein + H+	-	?
NAD+ + (ADP-D-ribosyl)n-RhoA protein	Paenibacillus larvae subsp. larvae BRL-230010	-	nicotinamide + (ADP-D-ribosyl)n+1-RhoA protein + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus larvae subsp. larvae	W2E3J5	-	-
Paenibacillus larvae subsp. larvae BRL-230010	W2E3J5	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap chelating Sepharose column chromatography, and Superdex 200 gel filtration	Paenibacillus larvae subsp. larvae

Source Tissue

Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + (ADP-D-ribosyl)n-RhoA protein	-	Paenibacillus larvae subsp. larvae	nicotinamide + (ADP-D-ribosyl)n+1-RhoA protein + H+	-	?
NAD+ + (ADP-D-ribosyl)n-RhoA protein	-	Paenibacillus larvae subsp. larvae BRL-230010	nicotinamide + (ADP-D-ribosyl)n+1-RhoA protein + H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 22000, calculated from amino acid sequence	Paenibacillus larvae subsp. larvae

Synonyms

Synonyms	Comment	Organism
ADP-ribosyltransferase	-	Paenibacillus larvae subsp. larvae
C3larvin toxin	-	Paenibacillus larvae subsp. larvae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.011	-	N-[[1-(1H-pyrazolo[3,4-d]pyrimidin-4-yl)piperidin-3-yl]methyl]methanesulfonamide	at pH 7.4 and 37°C	Paenibacillus larvae subsp. larvae

pI Value

Organism	Comment	pI Value Maximum	pI Value
Paenibacillus larvae subsp. larvae	calculated from amino acid sequence	-	9.6

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)