Cloned (Comment) | Organism |
---|---|
mutant C212S is expressed in Escherichia coli JM110 cells | Shewanella oneidensis |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme C212S, hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 0.1 M Tris pH 8.5, and 25% (w/v) PEG 3350 | Shewanella oneidensis |
Protein Variants | Comment | Organism |
---|---|---|
C212S | the mutation accounts for the lower affinity of the mutant for inorganic phosphate, while the affinity for uridine remains unchanged | Shewanella oneidensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
uridine | wild type enzyme, at pH 7.4 and 37°C | Shewanella oneidensis | |
0.24 | - |
uridine | mutant enzyme C212S, at pH 7.4 and 37°C | Shewanella oneidensis | |
5.1 | - |
phosphate | wild type enzyme, at pH 7.4 and 37°C | Shewanella oneidensis | |
16.1 | - |
phosphate | mutant enzyme C212S, at pH 7.4 and 37°C | Shewanella oneidensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uridine + phosphate | Shewanella oneidensis | - |
uracil + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | Shewanella oneidensis MR-1 / ATCC 700550 | - |
uracil + alpha-D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella oneidensis | C7EWM3 | - |
- |
Shewanella oneidensis MR-1 / ATCC 700550 | C7EWM3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Shewanella oneidensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uridine + phosphate | - |
Shewanella oneidensis | uracil + alpha-D-ribose 1-phosphate | - |
? | |
uridine + phosphate | - |
Shewanella oneidensis MR-1 / ATCC 700550 | uracil + alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | - |
Shewanella oneidensis |