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Literature summary for 2.4.2.3 extracted from

  • Zhu, S.; Song, D.; Gong, C.; Tang, P.; Li, X.; Wang, J.; Zheng, G.
    Biosynthesis of nucleoside analogues via thermostable nucleoside phosphorylase (2013), Appl. Microbiol. Biotechnol., 97, 6769-6778.
    View publication on PubMed
Search for more literature for 2.4.2.3

Application

Application Comment Organism
synthesis the enzyme is a valuable industrial biocatalysts for high-temperature reactions that produce nucleoside drugs in high yields Aeropyrum pernix

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, the native apUP protein without fusion tags is actively expressed, and about 50% of the protein is found in the soluble fraction of the cell lysate Aeropyrum pernix

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.248
-
 5-methyluridine pH 7.0, 90°C Aeropyrum pernix
0.615
-
 uridine pH 7.0, 90°C Aeropyrum pernix

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30200
-
 6 * 30200, calculated from sequence Aeropyrum pernix
185300
-
 gel filtration Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YA34
-
-
Aeropyrum pernix DSM 11879 Q9YA34
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aeropyrum pernix

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-methyluridine + phosphate 92% compared to the activity with uridine Aeropyrum pernix 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
5-methyluridine + phosphate 92% compared to the activity with uridine Aeropyrum pernix DSM 11879 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
deoxyuridine + phosphate 66% compared to the activity with uridine Aeropyrum pernix uracil + deoxy-D-ribose 1-phosphate
-
 ?
deoxyuridine + phosphate 66% compared to the activity with uridine Aeropyrum pernix DSM 11879 uracil + deoxy-D-ribose 1-phosphate
-
 ?
thymidine + phosphate 60% compared to the activity with uridine Aeropyrum pernix thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
thymidine + phosphate 60% compared to the activity with uridine Aeropyrum pernix DSM 11879 thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
uridine + phosphate the optimal substrate for the enzyme is uridine. It shows no activity for 5-fluorouridine, cytidine, or 2-deoxycytidine Aeropyrum pernix uracil + alpha-D-ribose 1-phosphate
-
 ?
uridine + phosphate the optimal substrate for the enzyme is uridine. It shows no activity for 5-fluorouridine, cytidine, or 2-deoxycytidine Aeropyrum pernix DSM 11879 uracil + alpha-D-ribose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 30200, calculated from sequence Aeropyrum pernix

Synonyms

Synonyms Comment Organism
apUP
-
 Aeropyrum pernix

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Aeropyrum pernix

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 after incubation for 60 min at 90 °C, the enzyme remains 100% active Aeropyrum pernix

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
56
-
 5-methyluridine pH 7.0, 90°C Aeropyrum pernix
150
-
 uridine pH 7.0, 90°C Aeropyrum pernix

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Aeropyrum pernix

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 pH stability ranging from pH 3.0 to pH 9.0 Aeropyrum pernix

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
230
-
 5-methyluridine pH 7.0, 90°C Aeropyrum pernix
240
-
 uridine pH 7.0, 90°C Aeropyrum pernix