Literature summary for 2.4.2.3 extracted from

Oliva, I.; Zuffi, G.; Orsini, G.; Tonon, G.; de Gioia, L.; Ghisotti, D.
Mutagenesis of Escherichia coli uridine phosphorylase by random pentapeptide insertions (2004), Enzyme Microb. Technol., 35, 309-314.

No PubMed abstract available

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Protein Variants

Protein Variants	Comment	Organism
C206_A207insGVPLC	inactive and insoluble mutant protein	Escherichia coli
E79-E80insGVPLE	inactive mutant protein	Escherichia coli
G174_R175insRGTPG	134% of the activity of the wild-type enzyme	Escherichia coli
I102_N103insGVPHI	inactive and insoluble mutant protein	Escherichia coli
I98_Q99insRGTPI	inactive and insoluble mutant protein	Escherichia coli
K181_G182insGGTPK	67% of the activity of the wild-type enzyme	Escherichia coli
L9_G10insGVPHL	139% of the activity of the wild-type enzyme	Escherichia coli
S159_D160insGGTPS	inactive mutant protein	Escherichia coli
S183_M184insMGYPS	54% of the activity of the wild-type enzyme	Escherichia coli
S183_M184insRGTPS	117% of the activity of the wild-type enzyme	Escherichia coli
T12_K13insGVPLT	132% of the activity of the wild-type enzyme	Escherichia coli
T52_W53insWGTPT	inactive mutant protein	Escherichia coli
T67_G68insGGTPT	inactive mutant protein	Escherichia coli
V192_M193insMGYPV	inactive mutant protein	Escherichia coli
V31_E32insGVPRV	inactive and insoluble mutant protein	Escherichia coli
V42_K43insKGYPV	as active as the wild-type enzyme	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
uridine + phosphate	-	Escherichia coli	uracil + alpha-D-ribose 1-phosphate	-	?

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Release 2023.1 (January 2023)