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Literature summary for 2.4.2.3 extracted from
- Uridine phosphorylase from Escherichia coli. Kinetic properties and mechanism (1976), Biochim. Biophys. Acta, 429, 352-358.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ribose 1-phosphate | product inhibition | Escherichia coli |  |
| Uracil | product inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.91 | - |
uridine | - |
Escherichia coli | |
| 2.9 | - |
phosphate | - |
Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
B-96, ATCC 13473, two types of enzyme, I and II | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phosphorylase type I | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| uridine + phosphate = uracil + alpha-D-ribose 1-phosphate | random mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.1 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -73°C, stable for at least 3 months, purified enzyme | Escherichia coli |
| -73°C, stable for at least 6 months, crude extract | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uridine + phosphate | - |
Escherichia coli | uracil + alpha-D-ribose 1-phosphate | - |
? | |
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