Cloned (Comment) | Organism |
---|---|
mutant expressed in Escherichia coli BL21(DE3) pLysS cells | Zymomonas mobilis |
Crystallization (Comment) | Organism |
---|---|
in complex with guanine, pre-queuine 0, and pre-queuine 1, hanging drop vapour diffusion method, in 100 mM MES, pH 5.5, 1 mM dithiothreitol, 13% (w/v) PEG 8000, 10% (v/v) dimethyl sulfoxide | Zymomonas mobilis |
Protein Variants | Comment | Organism |
---|---|---|
E235Q | the mutation has no significant influence on kcat, but Km for preQ1 is drastically increased, while Km for preQ0 seems to be decreased | Zymomonas mobilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
pre-queuine 0 | mutant enzyme E235Q, Km less than 0.0005 mM, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
0.0007 | - |
pre-queuine 1 | wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
0.0009 | - |
tRNA guanine | wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
0.0009 | - |
pre-queuine 0 | wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
0.001 | - |
tRNA guanine | mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
0.0012 | - |
guanine | wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
0.0033 | - |
guanine | mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis | |
0.032 | - |
pre-queuine 1 | mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 | Zymomonas mobilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zymomonas mobilis | P28720 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine | prefers pre-queuine 1 over pre-queuine 0 | Zymomonas mobilis | 7-aminomethyl-7-carbaguanine34 in tRNA + guanine | - |
? | |
pre-queuine 0 + tRNA guanine | - |
Zymomonas mobilis | guanine + tRNA pre-queuine 0 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TGT | - |
Zymomonas mobilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
pre-queuine 0 | mutant enzyme E235Q, Km less than 0.0005 mM | Zymomonas mobilis | |
0.012 | - |
pre-queuine 0 | wild type enzyme | Zymomonas mobilis | |
0.054 | - |
tRNA guanine | wild type enzyme | Zymomonas mobilis | |
0.056 | - |
guanine | wild type enzyme | Zymomonas mobilis | |
0.07 | - |
tRNA guanine | mutant enzyme E235Q | Zymomonas mobilis | |
0.076 | - |
guanine | mutant enzyme E235Q | Zymomonas mobilis | |
0.1 | - |
pre-queuine 1 | mutant enzyme E235Q | Zymomonas mobilis | |
0.102 | - |
pre-queuine 1 | wild type enzyme | Zymomonas mobilis |