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Literature summary for 2.4.2.29 extracted from

  • Tidten, N.; Stengl, B.; Heine, A.; Garcia, G.A.; Klebe, G.; Reuter, K.
    Glutamate versus glutamine exchange swaps substrate selectivity in tRNA-guanine transglycosylase: insight into the regulation of substrate selectivity by kinetic and crystallographic studies (2007), J. Mol. Biol., 374, 764-776.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
mutant expressed in Escherichia coli BL21(DE3) pLysS cells Zymomonas mobilis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with guanine, pre-queuine 0, and pre-queuine 1, hanging drop vapour diffusion method, in 100 mM MES, pH 5.5, 1 mM dithiothreitol, 13% (w/v) PEG 8000, 10% (v/v) dimethyl sulfoxide Zymomonas mobilis

Protein Variants

Protein Variants Comment Organism
E235Q the mutation has no significant influence on kcat, but Km for preQ1 is drastically increased, while Km for preQ0 seems to be decreased Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
pre-queuine 0 mutant enzyme E235Q, Km less than 0.0005 mM, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 Zymomonas mobilis
0.0007
-
pre-queuine 1 wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 Zymomonas mobilis
0.0009
-
tRNA guanine wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 Zymomonas mobilis
0.0009
-
pre-queuine 0 wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 Zymomonas mobilis
0.001
-
tRNA guanine mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 Zymomonas mobilis
0.0012
-
guanine wild type enzyme, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 Zymomonas mobilis
0.0033
-
guanine mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 Zymomonas mobilis
0.032
-
pre-queuine 1 mutant enzyme E235Q, in 200 mM HEPES, pH 7.3, 20 mM MgCl2 Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis P28720
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine prefers pre-queuine 1 over pre-queuine 0 Zymomonas mobilis 7-aminomethyl-7-carbaguanine34 in tRNA + guanine
-
?
pre-queuine 0 + tRNA guanine
-
Zymomonas mobilis guanine + tRNA pre-queuine 0
-
?

Synonyms

Synonyms Comment Organism
TGT
-
Zymomonas mobilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0006
-
pre-queuine 0 mutant enzyme E235Q, Km less than 0.0005 mM Zymomonas mobilis
0.012
-
pre-queuine 0 wild type enzyme Zymomonas mobilis
0.054
-
tRNA guanine wild type enzyme Zymomonas mobilis
0.056
-
guanine wild type enzyme Zymomonas mobilis
0.07
-
tRNA guanine mutant enzyme E235Q Zymomonas mobilis
0.076
-
guanine mutant enzyme E235Q Zymomonas mobilis
0.1
-
pre-queuine 1 mutant enzyme E235Q Zymomonas mobilis
0.102
-
pre-queuine 1 wild type enzyme Zymomonas mobilis