Literature summary for 2.4.2.28 extracted from

Oh, S.I.; Park, J.; Yoon, S.; Kim, Y.; Park, S.; Ryu, M.; Nam, M.J.; Ok, S.H.; Kim, J.K.; Shin, J.S.; Kim, K.N.
The Arabidopsis calcium sensor calcineurin B-like 3 inhibits the 5'-methylthioadenosine nucleosidase in a calcium-dependent manner (2008), Plant Physiol., 148, 1883-1896.

Cloned(Commentary)

Cloned (Comment)	Organism
different regions of MTAN cloned into the pGBT9.BS vector and transformed into Y190 yeast cells carrying pGAD.GH or pGAD.CBL3. Expressed in Escherichia coli BL21 cells carrying a GST fusion construct. MTAN promoter-GUS construct (pBI.tMTAN) transformed into Agrobacterium tumefaciens strain GV3101 and introduced into Arabidopsis plants by the floral dip method. 35S::CFP-CBL3-Myc and 35S::YFP-MTANHA constructs introduced, either alone or in combination, into Nicotiana benthamiana leaves by Agrobacterium tumefaciens-mediated infiltration method. CBL3-GFP and MTAN-GFP chimeric genes under the control of the cauliflower mosaic virus 35S promoter (pMD.CBL3 and pMD.MTAN) transiently expressed in Allium cepa epidermal cells. Onion epidermal cells bombarded with fusion constructs CBL3-YN and MTAN-YC	Arabidopsis thaliana

Cloned (Comment)

Organism

different regions of MTAN cloned into the pGBT9.BS vector and transformed into Y190 yeast cells carrying pGAD.GH or pGAD.CBL3. Expressed in Escherichia coli BL21 cells carrying a GST fusion construct. MTAN promoter-GUS construct (pBI.tMTAN) transformed into Agrobacterium tumefaciens strain GV3101 and introduced into Arabidopsis plants by the floral dip method. 35S::CFP-CBL3-Myc and 35S::YFP-MTANHA constructs introduced, either alone or in combination, into Nicotiana benthamiana leaves by Agrobacterium tumefaciens-mediated infiltration method. CBL3-GFP and MTAN-GFP chimeric genes under the control of the cauliflower mosaic virus 35S promoter (pMD.CBL3 and pMD.MTAN) transiently expressed in Allium cepa epidermal cells. Onion epidermal cells bombarded with fusion constructs CBL3-YN and MTAN-YC

Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
calcineurin B-like 3	inhibits only in the presence of Ca2+, MTAN interacts specifically with calcineurin B-like 3, but not with calcineurin B-like 1 and calcineurin B-like 4. The 91-amino acid region of calcineurin B-like 3 is sufficient for the interaction with MTAN. Calcineurin B-like 3 and MTAN associate with each other in plant cells, they interact in vitro and in vivo, form a complex outside of the nucleus, colocalize mainly at the plasma membrane	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
additional information	localized at the plasma membrane, in the cytoplasm and in the nucleus of transgenic tobacco plants. Abundant in the membrane structure and weak in the cytoplasm of onion epidermal cells	Arabidopsis thaliana	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	-	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9T0I8	-	-

Purification (Commentary)

Purification (Comment)	Organism
as a GST fusion form, by gel filtration	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
cauline leaf	-	Arabidopsis thaliana	-
flower	highest level	Arabidopsis thaliana	-
root	-	Arabidopsis thaliana	-
rosette leaf	-	Arabidopsis thaliana	-
stem	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5'-methylthioadenosine + phosphate	-	Arabidopsis thaliana	adenine + 5-methylthio-D-ribose 1-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
5'-methylthioadenosine nucleosidase	-	Arabidopsis thaliana
MTAN	-	Arabidopsis thaliana

General Information

General Information	Comment	Organism
malfunction	none of MTAN deletion mutants interact with calcineurin B-like 3	Arabidopsis thaliana
physiological function	MTAN is involved in pollen development	Arabidopsis thaliana