Cloned (Comment) | Organism |
---|---|
quantitative PCR, RNA mutational analysis, analysis of methylation status of the enzymes promotor | Homo sapiens |
re-expression in Mel Im cell line | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | melanoma cell line HTZ19d is a deletion mutant without enzyme expression and activity | Homo sapiens |
V56I | natural polymorphism present in 7 of 9 melanoma cell lines, not in SK-Mel-28 and in HTZ19d | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | promotor hypermethylation strongly reduces enzyme expression | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | inverse correlation between enzyme expression and progression of melanocytic tumors, important role of enzyme inactivation in the development of melanomas | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HMB-2 cell | cell line derived from metastases of malignant melanomas | Homo sapiens | - |
Mel Ei cell | cell line derived from primary cutaneous melanoma | Homo sapiens | - |
Mel Im cell | cell line derived from metastases of malignant melanomas | Homo sapiens | - |
Mel Ju cell | cell line derived from metastases of malignant melanomas | Homo sapiens | - |
Mel Wei cell | cell line derived from primary cutaneous melanoma | Homo sapiens | - |
Mel-HO cell | low content | Homo sapiens | - |
Mel-HO cell | cell line derived from primary cutaneous melanoma | Homo sapiens | - |
MEL-JUSO cell | low content | Homo sapiens | - |
MEL-JUSO cell | cell line derived from primary cutaneous melanoma | Homo sapiens | - |
melanocyte | primary | Homo sapiens | - |
melanoma cell | reduced activity due to hypermethylation of the promotor | Homo sapiens | - |
melanoma cell line | SK-Ml-28 cell line derived from metastases of malignant melanomas | Homo sapiens | - |
additional information | strongly reduced expression in all melanoma cell lines due to promotor hypermethylation, no enzyme content and expression in melanoma cell line HTZ19d | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + phosphate | - |
Homo sapiens | adenine + 5-methylthio-D-ribose 1-phosphate | - |
? | |
additional information | inverse correlation between enzyme expression and progression of melanocytic tumors, important role of enzyme inactivation in the development of melanomas | Homo sapiens | ? | - |
? |