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Literature summary for 2.4.2.28 extracted from

  • Appleby, T.C.; Erion, M.D.; Ealick, S.E.
    The structure of human 5'-deoxy-5'-methylthioadenosine phosphorylase at 1.7 A resolution provides insights into substrate binding and catalysis (1999), Structure, 7, 629-641.
    View publication on PubMed
Search for more literature for 2.4.2.28

Application

Application Comment Organism
medicine enzyme is a potential chemotherapeutic target Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged protein in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
from recombinant enzyme, hanging drop-vapour diffusion method, protein 25 mg/ml, 10 mM Tris-HCl, pH 7.4, 5 mM DTT, 0.4 M NaCl against reservoir solution of 80 mM MES, pH 5.9, 5 mM DTT, 12% w/v polyethylene glycol 6000, 25% v/v ethylene glycol, in presence of substrates adenine, phosphate and sulfate, structure analysis by X-ray diffraction at light scattering Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-deoxy-5'-methylthioadenosine + phosphate Homo sapiens salvage reaction adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
5'-deoxy-5'-methylthioadenosine + phosphate Homo sapiens substrate is a by-product of polyamine biosynthesis, which is essential for cell growth and proliferation adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate catalytic mechanism Homo sapiens
a
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate active and substrate-binding sites, three-dimensional structures Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-deoxy-5'-methylthioadenosine + phosphate sugar specificity Homo sapiens adenine + 5-methylthio-D-ribose 1-phosphate
-
 r
5'-deoxy-5'-methylthioadenosine + phosphate salvage reaction Homo sapiens adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
5'-deoxy-5'-methylthioadenosine + phosphate substrate is a by-product of polyamine biosynthesis, which is essential for cell growth and proliferation Homo sapiens adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
More overall and active site structure, trimer of 3 identical subunits Homo sapiens
More base- and methylribose-binding sites Homo sapiens