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Literature summary for 2.4.2.28 extracted from

  • Della Ragione, F.; Takabayashi, K.; Mastropietro, S.; Mercurio, C.; Oliva, A.; Russo, G.L.; Della Pietra, V.; Borriello, A.; Nobori, T.; et al.
    Purification and characterization of recombinant human 5'-methylthioadenosine phosphorylase: definite identification coding cDNA (1996), Biochem. Biophys. Res. Commun., 223, 514-519.
    View publication on PubMed
Search for more literature for 2.4.2.28

Application

Application Comment Organism
medicine gene is linked to the important tumor suppressor gene p16INK4A, enzyme is of key importance in defining the chromosomal area homozygously deleted in a large number of human tumors, absence of enzyme activity only in malignant cells is of great value in developing selective antitumor therapeutic strategies Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
the gene maps on the 9p21 chromosome, strictly linked to the tumor suppressor gene p16INK4A, functional expression in Escherichia coli strain JM105 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5'-Chloroformycin
-
 Homo sapiens
5'-methylthiotubercidin
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 5'-methylthioadenosine recombinant enzyme Homo sapiens
0.75
-
 phosphate recombinant enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 3 * 30000, recombinant enzyme, SDS-PAGE Homo sapiens
90000
-
 recombinant enzyme, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.5
-
 purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + phosphate
-
 Homo sapiens adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 30000, recombinant enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens