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Literature summary for 2.4.2.28 extracted from

  • Carteni'-Farina, M.; Oliva, A.; Romeo, G.; Napolitano, G.; De Rosa, M.; Gambacorta, A.; Zappia, V.
    5'-Methylthioadenosine phosphorylase from Caldariella acidophila. Purification and properties (1979), Eur. J. Biochem., 101, 317-324.
    View publication on PubMed
Search for more literature for 2.4.2.28

Inhibitors

Inhibitors Comment Organism Structure
additional information no effect by alkylating, mercaptide-forming or oxidizing thiol reagents Caldariella acidophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.095
-
 5'-methylthioadenosine
-
 Caldariella acidophila
6.1
-
 phosphate
-
 Caldariella acidophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-methylthioadenosine + phosphate Caldariella acidophila involved in salvage of adenine and methionine from 5'-methylthioadenosine adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
5'-methylthioadenosine + phosphate Caldariella acidophila physiological significance of 5'-methylthioadenosine cleavage is probably related to removal of the thioether which in turn exerts a significant inhibition on methyl transfer reactions adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Caldariella acidophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Caldariella acidophila

Reaction

Reaction Comment Organism Reaction ID
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate phosphorolytic mechanism Caldariella acidophila
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.48
-
 purified enzyme Caldariella acidophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-isobutylthioadenosine + phosphate 97% of the reaction with 5'-methylthioadenosine Caldariella acidophila adenine + 5-isobutylthio-D-ribose 1-phosphate
-
 ?
5'-isobutylthioinosine + phosphate 8.1% of the reaction with 5'-methylthioadenosine Caldariella acidophila hypoxanthine + 5-isobutylthio-D-ribose 1-phosphate
-
 ?
5'-methylthioadenosine + phosphate
-
 Caldariella acidophila adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
5'-methylthioadenosine + phosphate involved in salvage of adenine and methionine from 5'-methylthioadenosine Caldariella acidophila adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
5'-methylthioadenosine + phosphate physiological significance of 5'-methylthioadenosine cleavage is probably related to removal of the thioether which in turn exerts a significant inhibition on methyl transfer reactions Caldariella acidophila adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
5'-methylthioinosine + phosphate 8.8% of the reaction with 5'-methylthioadenosine Caldariella acidophila hypoxanthine + 5-methylthio-alpha-D-ribose 1-phosphate
-
 ?
5'-n-butylthioadenosine + phosphate 93.3% of the reaction with 5'-methylthioadenosine Caldariella acidophila adenine + 5-n-butylthio-D-ribose 1-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Caldariella acidophila
95
-
-
 Caldariella acidophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
86 95 86°C: 50% of activity maximum, 95°C: activity maximum, no activity at 40°C Caldariella acidophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 1 h, stable Caldariella acidophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.2 Caldariella acidophila
7.2
-
-
 Caldariella acidophila

pH Range

pH Minimum pH Maximum Comment Organism
6 9 50% of activity maximum at pH 6.0 and pH 9.0 Caldariella acidophila