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Literature summary for 2.4.2.26 extracted from

  • Mueller, B.; Prante, C.; Kleesiek, K.; Goetting, C.
    Identification and characterization of the human xylosyltransferase I gene promoter region (2009), J. Biol. Chem., 284, 30775-30782.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information the c-Jun/activator protein-1 transcription factor is essential for full XYLT1 promoter activity in SW1353 cells Homo sapiens

Application

Application Comment Organism
medicine ways of modulating the XYLT1 gene expression, especially in the development of therapeutic strategies for the treatment of fibrotic remodeling processes or cartilage repair Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
5' and 3' deletion constructs expressed in SW1353 cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Synonyms

Synonyms Comment Organism
xylosyltransferase I
-
Homo sapiens
XYLT1
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Homo sapiens

Expression

Organism Comment Expression
Homo sapiens SW1353 cells treated with curcumin (0.02 mM) and tanshinone IIA (0.1 mM), respectively, for 6 h show significantly reduced XT-I mRNA expression to 41.8% and 13.8%, respectively. SW1353 cells treated with different concentrations (10 nM to 0.001 mM) of mithramycin A for 24 h show a highly significant decrease of XT-I mRNA levels compared with nontreated cells. mRNA level of both specificity protein 1 and specificity protein 3 is significantly reduced to less than 30% compared with controls treated with a scrambled siRNA for a total of 96 h after transfection. The siRNA-mediated knockdown of specificity protein 1 has no detectable effect on the XT-I mRNA levels, whereas the knockdown of specificity protein 3 leads to a highly significant reduction to ca. 40% compared with controls. Decrease of 51% in the enzyme xylosyltransferase activity in the cell culture supernatant of specificity protein 3 siRNA-transfected cells compared with controls 96 h after transfection down
Homo sapiens members of the activator protein 1 and specificity protein 1 family of transcription factors are necessary for the transcriptional regulation of the XYLT1 gene. Transcription factors of the specificity protein 1 family, especially specificity protein 3, are very likely involved in the regulation of the XT-I mRNA expression. A 531-bp core promoter element is able to drive the transcription on a basal level. A binding site for transcription factors of the activator protein 1 family, which is essential for full promoter activity is located 730 bp 5' of the translation initiation site. A promoter element containing this binding site is able to drive the transcription to about 79fold above control in SW1353 chondrosarcoma cells up