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Literature summary for 2.4.2.26 extracted from

  • Götting, C.; Müller, S.; Schöttler, M.; Schön, S.; Prante, C.; Brinkmann, T.; Kuhn, J.; Kleesiek, K.
    Analysis of the DXD motifs in human xylosyltransferase I required for enzyme activity (2004), J. Biol. Chem., 279, 42566-42573.
    View publication on PubMed
Search for more literature for 2.4.2.26

Protein Variants

Protein Variants Comment Organism
D314G 80% of the activity of wild-type enzyme,Km-value for bikunin is 1.2fold higher than wild-type value Homo sapiens
D316G as active as wild-type enzyme, Km-value for bikunin is identical to wild-type value Homo sapiens
D745E mutant retains full activity, Km-value for bikunin is 1.2fold higher than wild-type value Homo sapiens
D745G loss of activity Homo sapiens
D747E reduced substrate affinity, about 35% of the wild-type activity, Km-value for bikunin is 4.4fold higher than wild-type value Homo sapiens
D747G reduced substrate affinity, about 35% of the wild-type activity, Km-value for bikunin is 7.7fold higher than wild-type value Homo sapiens
W746D about 25% of wild-type activity, Km-value for bikunin is 1.3fold higher than wild-type value Homo sapiens
W746G about 25% of wild-type activity, Km-value for bikunin is 1.3fold higher than wild-type value Homo sapiens
W746N about 25% of wild-type activity, Km-value for bikunin is 1.4fold higher than wild-type value Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
heparin
-
 Homo sapiens
UDP
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 bikunin pH 6.5, 37°C, wild-type enzyme Homo sapiens
0.0009
-
 bikunin pH 6.5, 37°C, mutant enzyme D316G Homo sapiens
0.0011
-
 bikunin pH 6.5, 37°C, mutant enzyme D314G Homo sapiens
0.0011
-
 bikunin pH 6.5, 37°C, mutant enzyme D745E Homo sapiens
0.0012
-
 bikunin pH 6.5, 37°C, mutant enzyme W746D Homo sapiens
0.0012
-
 bikunin pH 6.5, 37°C, mutant enzyme W746G Homo sapiens
0.0013
-
 bikunin pH 6.5, 37°C, mutant enzyme W746N Homo sapiens
0.0044
-
 bikunin pH 6.5, 37°C, mutant enzyme D747E Homo sapiens
0.0069
-
 bikunin pH 6.5, 37°C, mutant enzyme D747G Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86Y38
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-xylose + bikunin
-
 Homo sapiens UDP + ?
-
 ?

Synonyms

Synonyms Comment Organism
XT-I
-
 Homo sapiens
xylosyltransferase I
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00007
-
 heparin pH 6.5, 37°C, mutant enzyme D745E Homo sapiens
0.00011
-
 heparin pH 6.5, 37°C, mutant enzyme D747E Homo sapiens
0.00011
-
 heparin pH 6.5, 37°C, mutant enzyme W746N Homo sapiens
0.00012
-
 heparin pH 6.5, 37°C, mutant enzyme W746G Homo sapiens
0.00012
-
 heparin pH 6.5, 37°C, wild-type enzyme Homo sapiens
0.00013
-
 heparin pH 6.5, 37°C, mutant enzyme W746D Homo sapiens
0.00014
-
 heparin pH 6.5, 37°C, mutant enzyme D316G Homo sapiens
0.00015
-
 heparin pH 6.5, 37°C, mutant enzyme D314G Homo sapiens
0.00015
-
 heparin pH 6.5, 37°C, mutant enzyme D747G Homo sapiens
0.0019
-
 UDP pH 6.5, 37°C, mutant enzyme D745E Homo sapiens
0.0072
-
 UDP pH 6.5, 37°C, mutant enzyme D314G Homo sapiens
0.0108
-
 UDP pH 6.5, 37°C, wild-type enzyme Homo sapiens
0.0113
-
 UDP pH 6.5, 37°C, mutant enzyme D316G Homo sapiens
0.0147
-
 UDP pH 6.5, 37°C, mutant enzyme D747E Homo sapiens
0.0172
-
 UDP pH 6.5, 37°C, mutant enzyme D747G Homo sapiens
0.0179
-
 UDP pH 6.5, 37°C, mutant enzyme W746N Homo sapiens
0.0196
-
 UDP pH 6.5, 37°C, mutant enzyme W746D Homo sapiens
0.0246
-
 UDP pH 6.5, 37°C, mutant enzyme W746G Homo sapiens