Any feedback?
Literature summary for 2.4.2.21 extracted from
- Vitamin B 12 biosynthesis. Enzyme studies on the formation of the alpha-glycosidic nucleotide precursor (1969), J. Biol. Chem., 244, 1659-1666.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
adenine | - |
Acetoanaerobium sticklandii |  |
| 0.3 | - |
nicotinic acid mononucleotide | cosubstrate adenine | Acetoanaerobium sticklandii | |
| 0.5 | - |
benzimidazole | - |
Acetoanaerobium sticklandii | |
| 0.7 | - |
nicotinic acid mononucleotide | cosubstrate benzimidazole | Acetoanaerobium sticklandii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetoanaerobium sticklandii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetoanaerobium sticklandii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate + H+ | single displacement mechanism | Acetoanaerobium sticklandii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
- |
Acetoanaerobium sticklandii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, 0.01 M potassium phosphate buffer, pH 6.8, 0.005 M DTT, 10% ethylene glycol, several months | Acetoanaerobium sticklandii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-nicotinate D-ribonucleotide + 5(6)-nitrobenzimidazole | - |
Acetoanaerobium sticklandii | nicotinate + N1-(5-phospho-alpha-D-ribosyl)-5(6)-nitrobenzimidazole | - |
? | |
| beta-nicotinate D-ribonucleotide + 5,6-dichlorobenzimidazole | - |
Acetoanaerobium sticklandii | nicotinate + N1-(5-phospho-alpha-D-ribosyl)-5,6-dichlorobenzimidazole | - |
? | |
| beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole | - |
Acetoanaerobium sticklandii | nicotinate + N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole | - |
? | |
| nicotinic acid mononucleotide + adenine | - |
Acetoanaerobium sticklandii | 7-alpha-D-ribofuranosyladenine 5'-phosphate + nicotinate | - |
? | |
| nicotinic acid mononucleotide + benzimidazole | highly specific for nicotinic acid mononucleotide | Acetoanaerobium sticklandii | 1-alpha-D-ribofuranosylbenzimidazole 5'-phosphate + nicotinate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Acetoanaerobium sticklandii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
assay at | Acetoanaerobium sticklandii |
| 8.6 | 9.2 | nicotinic acid mononucleotide + adenine or benzimidazole | Acetoanaerobium sticklandii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
