Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method at 16°C, the apocrystals are obtained using 0.1 M buffer MES pH 6.5 and magnesium sulfate as precipitant, crystals in the presence of AMP and histidine are obtained using 0.1 M sodium citrate pH 5.6, 0.5 M ammonium sulfate and 1 M lithium sulfate with 5 mM AMP and 0.1 mM histidine | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-histidine | allosteric inhibition, synergistically favored by AMP; feed-back inhibition | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-nitrilotriacetic acid column, Sephadex G-200 later on | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | 1-(5-phospho-D-ribosyl)-ATP + diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | active form | Mycobacterium tuberculosis |
hexamer | inactive form, in complex with histidine | Mycobacterium tuberculosis |