Literature summary for 2.4.2.10 extracted from

Kumar, S.; Krishnamoorthy, K.; Mudeppa, D.G.; Rathod, P.K.
Structure of Plasmodium falciparum orotate phosphoribosyltransferase with autologous inhibitory protein-protein interactions (2015), Acta Crystallogr. Sect. F, 71, 600-608.

Search for more literature for 2.4.2.10

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JW3617(DE3) cells	Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, using 100 mM ammonium sulfate, 30% (w/v) PEG 3350, at 4°C	Plasmodium falciparum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate	Plasmodium falciparum	-	orotidine 5'-phosphate + diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q8N0R1	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography, HiTrap Q-Sepharose column chromatography, and S200 XK gel filtration	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate	-	Plasmodium falciparum	orotidine 5'-phosphate + diphosphate	-	r

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
OPRTase	-	Plasmodium falciparum

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Release 2023.1 (January 2023)