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Literature summary for 2.4.2.10 extracted from

  • Zhang, Y.; Luo, M.; Schramm, V.L.
    Transition states of Plasmodium falciparum and human orotate phosphoribosyltransferases (2009), J. Am. Chem. Soc., 131, 4685-4694.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed from pDEST14-OPRT in Escherichia coli BL21-AI competent cells Homo sapiens
into the pDONR221 vector, expressed from pDEST14-OPRT in Escherichia coli BL21-AI competent cells Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
p-nitrophenyl beta-D-ribose
-
 Homo sapiens
p-nitrophenyl beta-D-ribose
-
 Plasmodium falciparum
p-nitrophenyl beta-D-ribose 5'-phosphate competitive inhibitor against orotidine 5'-phosphate Homo sapiens
p-nitrophenyl beta-D-ribose 5'-phosphate competitive inhibitor against orotidine 5'-phosphate Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00155
-
 orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
0.00214
-
 orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
0.0029
-
 orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
0.00373
-
 orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
0.091
-
 Orotidine
-
 Homo sapiens
0.096
-
 Orotidine
-
 Plasmodium falciparum
1.59
-
 Phosphonoacetic acid
-
 Plasmodium falciparum
3.84
-
 Phosphonoacetic acid
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens
Mg2+
-
 Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+ affinity column Homo sapiens
Ni2+ affinity column Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens orotidine 5'-phosphate + diphosphate
-
 ?
orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum orotidine 5'-phosphate + diphosphate
-
 ?
orotidine 5'-phosphate + diphosphate
-
 Homo sapiens orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
orotidine 5'-phosphate + diphosphate
-
 Plasmodium falciparum orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
orotidine 5'-phosphate + phosphonoacetic acid
-
 Homo sapiens ?
-
 ?
orotidine 5'-phosphate + phosphonoacetic acid
-
 Plasmodium falciparum ?
-
 ?

Synonyms

Synonyms Comment Organism
OPRT
-
 Homo sapiens
OPRT
-
 Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
 orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
0.017
-
 Phosphonoacetic acid
-
 Homo sapiens
0.017
-
 orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
0.023
-
 Phosphonoacetic acid
-
 Plasmodium falciparum
0.024
-
 Orotidine
-
 Homo sapiens
0.042
-
 Orotidine
-
 Plasmodium falciparum
0.1
-
 orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
1.96
-
 orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00004
-
 p-nitrophenyl beta-D-ribose 5'-phosphate in the presence of orotidine 5'-phosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
0.000041
-
 p-nitrophenyl beta-D-ribose 5'-phosphate in the presence of orotidine 5'-phosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
0.000121
-
 p-nitrophenyl beta-D-ribose in the presence of orotidine 5'-phosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
0.000188
-
 p-nitrophenyl beta-D-ribose in the presence of orotidine 5'-phosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.8
-
 orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum
7.9
-
 orotidine 5'-phosphate in the presence of 20 mM phosphonoacetic acid, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
65
-
 orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Homo sapiens
530
-
 orotidine 5'-phosphate in the presence of 1 mM diphosphate, at 25°C, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2 Plasmodium falciparum