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Literature summary for 2.4.2.10 extracted from

  • Jones, M.E.; Kavipurapu, P.R.; Traut, T.W.
    Orotate phosphoribosyltransferase: orotidylate decarboxylase (Ehrlich ascites cell) (1978), Methods Enzymol., 51, 155-167.
    View publication on PubMed
Search for more literature for 2.4.2.10

General Stability

General Stability Organism
5 mM Mg2+, 1 mM phosphoribose diphosphate and 2 mM DTT stabilize dilute enzyme solutions Mus musculus
dialysis against phosphate buffer, pH 7 or 7.5, unstable to, 2 mM DTT protect Mus musculus
dilution inactivates Mus musculus
phosphate, 0.3 M and above, stabilizes Mus musculus
proteins, e.g. albumin, do not stabilize dilute enzyme solutions Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
5-Bromouracil
-
 Mus musculus
5-Chlorouracil
-
 Mus musculus
5-Fluoroorotate at 0.05 M Mus musculus
adenine
-
 Mus musculus
adenosine
-
 Mus musculus
ADP
-
 Mus musculus
allopurinol
-
 Mus musculus
AMP
-
 Mus musculus
ATP
-
 Mus musculus
Azauracil
-
 Mus musculus
Azauridine
-
 Mus musculus
Barbituric acid
-
 Mus musculus
cytosine
-
 Mus musculus
dihydroorotate 10 mM Mus musculus
diphosphate
-
 Mus musculus
dUMP
-
 Mus musculus
GMP
-
 Mus musculus
IMP
-
 Mus musculus
additional information not inhibitory: CMP, TMP, 6-aza-UMP or 5-bromo-UMP Mus musculus
Orotidine 10 mM Mus musculus
Oxipurinol
-
 Mus musculus
phosphate
-
 Mus musculus
ribose 5-phosphate not inhibitory Mus musculus
UDP
-
 Mus musculus
UMP 1 mM, not 0.1 mM Mus musculus
Uracil
-
 Mus musculus
uridine
-
 Mus musculus
UTP
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information principles of assay Mus musculus
0.002
-
 Orotate
-
 Mus musculus
0.016
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Mus musculus
Mg2+ Km-value: 3 mM Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.115
-
-
 Mus musculus

Storage Stability

Storage Stability Organism
-60°C to-20°C, crude enzyme or ammonium sulfate preparation, 2 years Mus musculus
frozen, partially purified enzyme in phosphate buffer, pH 7 or 7.5, 2 months Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Mus musculus orotidine 5'-phosphate + diphosphate i.e. orotidylate or OMP ?

Synonyms

Synonyms Comment Organism
More constitutes together with orotidylate decarboxylase (EC 4.1.1.23) UMP-synthase, former complex U or multienzyme pyr-5,6 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 preferred buffer: Tris-HCl, when substrates are non-saturating, phosphate buffer, when substrate are saturating, not: maleate, imidazole, N-tris[hydroxymethyl] methyl-2-aminoethane-sulfonic acid (i.e. TES) or 3-[N-morpholino]-2-hydroxypropanesulfonic acid (i.e. MOPS) buffer Mus musculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 stable Mus musculus