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Literature summary for 2.4.2.1 extracted from

  • Donaldson, T.M.; Cassera, M.B.; Ho, M.C.; Zhan, C.; Merino, E.F.; Evans, G.B.; Tyler, P.C.; Almo, S.C.; Schramm, V.L.; Kim, K.
    Inhibition and structure of Toxoplasma gondii purine nucleoside phosphorylase (2014), Eukaryot. Cell, 13, 572-579.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-Codon plus cells Plasmodium falciparum
expressed in Escherichia coli BL21-Codon plus cells Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with phosphate and immucillin-H, sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol monomethyl ether 2000, 100 mM Tris (pH 8.5), and 0.2 M trimethylamine N-oxide dihydrate Plasmodium falciparum
in complex with phosphate and immucillin-H, sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol monomethyl ether 2000, 100 mM Tris (pH 8.5), and 0.2 M trimethylamine N-oxide dihydrate Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
1',9-methyl-immucillin-H
-
 Plasmodium falciparum
1',9-methyl-immucillin-H
-
 Toxoplasma gondii
2'-deoxy-immucillin-H
-
 Plasmodium falciparum
2'-deoxy-immucillin-H
-
 Toxoplasma gondii
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G
-
 Plasmodium falciparum
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G
-
 Toxoplasma gondii
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H
-
 Plasmodium falciparum
4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H
-
 Toxoplasma gondii
5'-amido-immucillin-H
-
 Plasmodium falciparum
5'-amido-immucillin-H
-
 Toxoplasma gondii
5'-carboxy-immucillin-H
-
 Plasmodium falciparum
5'-carboxy-immucillin-H
-
 Toxoplasma gondii
5'-deoxy-immucillin-H
-
 Plasmodium falciparum
5'-deoxy-immucillin-H
-
 Toxoplasma gondii
5'-fluoro-immucillin-H
-
 Plasmodium falciparum
5'-fluoro-immucillin-H
-
 Toxoplasma gondii
5'-methylthio-immucillin-H
-
 Plasmodium falciparum
5'-methylthio-immucillin-H
-
 Toxoplasma gondii
5'-thio-immucillin-H
-
 Plasmodium falciparum
5'-thio-immucillin-H
-
 Toxoplasma gondii
immucillin-H transition-state analogue inhibitor Plasmodium falciparum
immucillin-H transition-state analogue inhibitor Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 Inosine pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.011
-
 Inosine pH 7.4, temperature not specified in the publication Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-
Toxoplasma gondii Q2HXR2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography, and gel filtration Plasmodium falciparum
Ni-NTA affinity column chromatography, and gel filtration Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
 Plasmodium falciparum
-
tachyzoite
-
 Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine + phosphate
-
 Plasmodium falciparum hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
inosine + phosphate
-
 Toxoplasma gondii hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Synonyms

Synonyms Comment Organism
PNP
-
 Plasmodium falciparum
PNP
-
 Toxoplasma gondii
purine nucleoside phosphorylase
-
 Plasmodium falciparum
purine nucleoside phosphorylase
-
 Toxoplasma gondii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
 Inosine pH 7.4, temperature not specified in the publication Toxoplasma gondii
1.7
-
 Inosine pH 7.4, temperature not specified in the publication Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000000093
-
 5'-thio-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.00000027
-
 5'-methylthio-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.00000037
-
 immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.0000005
-
 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.00000086
-
 immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.0000009
-
 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.0000011
-
 5'-deoxy-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.0000016
-
 5'-deoxy-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.0000019
-
 2'-deoxy-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.000002
-
 5'-fluoro-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.0000022
-
 5'-fluoro-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.000012
-
 5'-carboxy-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.000017
-
 5'-carboxy-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.000019
-
 2'-deoxy-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.00022
-
 5'-amido-immucillin-H pH 7.4, temperature not specified in the publication Plasmodium falciparum
0.0015
-
 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-G pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.0036
-
 4'-deaza-1'-aza-2'-deoxy-1',9-methyl-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.0056
-
 5'-methylthio-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.0089
-
 5'-amido-immucillin-H pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.05
-
 5'-thio-immucillin-H Ki above 0.05 mM, pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.05
-
 1',9-methyl-immucillin-H Ki above 0.05 mM, pH 7.4, temperature not specified in the publication Toxoplasma gondii
0.6
-
 1',9-methyl-immucillin-H Ki above 0.6 mM, pH 7.4, temperature not specified in the publication Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15
-
 Inosine pH 7.4, temperature not specified in the publication Plasmodium falciparum
55
-
 Inosine pH 7.4, temperature not specified in the publication Toxoplasma gondii