Inhibitors | Comment | Organism | Structure |
---|---|---|---|
9-benzylguanine | isothermal titration calorimetry study. At 25°C, the tighter 9-benzylguanine binding reaction goes from an enthalpically-driven reaction in the absence of phosphate to an entropically-driven reaction at 10 mM phosphate, and the enthalpically-driven nature of the binding reaction is restored at 75 mM phosphate. Bound phosphate affects the interactions of the side-chains with the ribose catalytic site. 9-Benzylguanine interacts with Phe159 from an adjacent subunit | Homo sapiens | |
acyclovir | isothermal titration calorimetry study. At 25°C and with an increase in the phosphate concentration from 0 to 50 mM, acyclovir binding becomes more entropically-driven | Homo sapiens | |
ganciclovir | isothermal titration calorimetry study. At 25°C and with an increase in the phosphate concentration from 0 to 50 mM, ganciclovir binding becomes more enthalpically-driven | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.2 | - |
7-methylguanosine | presence of 50 mM phosphate, pH 7.0 | Homo sapiens | |
12.5 | - |
7-methylguanosine | presence of 50 mM phosphate, pH 7.0 | Homo sapiens | |
13 | - |
7-methylguanosine | presence of 100 mM phosphate, pH 7.0 | Homo sapiens | |
13.9 | - |
7-methylguanosine | presence of 100 mM phosphate, pH 7.0 | Homo sapiens | |
16.3 | - |
7-methylguanosine | presence of 100 mM phosphate, pH 7.0 | Homo sapiens | |
39.3 | - |
7-methylguanosine | presence of 50 mM phosphate, pH 7.0 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12 | - |
presence of 100 mM phosphate, pH 7.0 | Homo sapiens |
20 | - |
presence of 50 mM phosphate, pH 7.0 | Homo sapiens |
41 | - |
presence of 50 mM phosphate, pH 7.0 | Homo sapiens |
46 | - |
presence of 100 mM phosphate, pH 7.0 | Homo sapiens |
47 | - |
presence of 100 mM phosphate, pH 7.0 | Homo sapiens |
139 | - |
presence of 50 mM phosphate, pH 7.0 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylguanosine + phosphate | - |
Homo sapiens | 7-methylguanine + alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | partial dissociation of the purine nucleotide phosphates trimer into monomers below 60 nM | Homo sapiens |
trimer | partial dissociation of the purine nucleotide phosphates trimer into monomers below 60 nM | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.23 | - |
7-methylguanosine | presence of 100 mM phosphate, pH 7.0 | Homo sapiens | |
9.2 | - |
7-methylguanosine | presence of 50 mM phosphate, pH 7.0 | Homo sapiens | |
20.4 | - |
7-methylguanosine | presence of 50 mM phosphate, pH 7.0 | Homo sapiens | |
23 | - |
7-methylguanosine | presence of 100 mM phosphate, pH 7.0 | Homo sapiens | |
24.7 | - |
7-methylguanosine | presence of 100 mM phosphate, pH 7.0 | Homo sapiens | |
73.1 | - |
7-methylguanosine | presence of 50 mM phosphate, pH 7.0 | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
240 | - |
7-methylguanosine | presence of 50 mM phosphate, pH 7.0 | Homo sapiens | |
488 | - |
7-methylguanosine | presence of 100 mM phosphate, pH 7.0 | Homo sapiens | |
1548 | - |
7-methylguanosine | presence of 100 mM phosphate, pH 7.0 | Homo sapiens | |
1674 | - |
7-methylguanosine | presence of 100 mM phosphate, pH 7.0 | Homo sapiens | |
2256 | - |
7-methylguanosine | presence of 50 mM phosphate, pH 7.0 | Homo sapiens | |
5860 | - |
7-methylguanosine | presence of 50 mM phosphate, pH 7.0 | Homo sapiens |