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Literature summary for 2.4.2.1 extracted from

  • Bennett, E.M.; Li, C.; Allan, P.W.; Parker, W.B.; Ealick, S.E.
    Structural basis for substrate specificity of Escherichia coli purine nucleoside phosphorylase (2003), J. Biol. Chem., 278, 47110-47118.
    View publication on PubMed
Search for more literature for 2.4.2.1

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method. Enzyme in complex with nucleoside analogs: 2-fluoroadenosine, 9-beta-D-ribofuranosyl-6-methylthiopurine, 2-fluoro-2'-deoxyadenosine, 9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine, adenosine, formycin B, 7-deazaadenosine, 9-beta-D-arabinofuranosyl-adenine, 9-beta-D-xylofuranosyl-adenine or inosine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 9-beta-D-ribofuranosyl-6-methylthiopurine pH 7.4, 25°C Escherichia coli
0.012
-
 2-fluoroadenosine pH 7.4, 25°C Escherichia coli
0.023
-
 2-fluoro-2'-deoxyadenosine pH 7.4, 25°C Escherichia coli
0.046
-
 adenosine pH 7.4, 25°C Escherichia coli
0.096
-
 Inosine pH 7.4, 25°C Escherichia coli
0.126
-
 9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine pH 7.4, 25°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0ABP8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-fluoro-2-deoxyadenosine + phosphate
-
 Escherichia coli adenine + 2-fluoro-2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2-fluoroadenosine + phosphate
-
 Escherichia coli 2-fluoroadenine + alpha-D-ribose 1-phosphate
-
 ?
9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine + phosphate
-
 Escherichia coli 6-methylthiopurine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
9-beta-D-ribofuranosyl-6-methylthiopurine + phosphate
-
 Escherichia coli 6-methylthiopurine + alpha-D-ribose 1-phosphate
-
 ?
adenosine + phosphate
-
 Escherichia coli adenine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 9-beta-D-ribofuranosyl-6-methylthiopurine pH 7.4, 25°C Escherichia coli
2.05
-
 2-fluoroadenosine pH 7.4, 25°C Escherichia coli
2.77
-
 2-fluoro-2'-deoxyadenosine pH 7.4, 25°C Escherichia coli
3.3
-
 adenosine pH 7.4, 25°C Escherichia coli
4
-
 Inosine pH 7.4, 25°C Escherichia coli
9.7
-
 9-beta-D-(2-deoxyribofuranosyl)-6-methylthiopurine pH 7.4, 25°C Escherichia coli