Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.1 extracted from

  • Lee, J.; Filosa, S.; Bonvin, J.; Guyon, S.; Aponte, R.A.; Turnbull, J.L.
    Expression, purification, and characterization of recombinant purine nucleoside phosphorylase from Escherichia coli (2001), Protein Expr. Purif., 22, 180-188.
    View publication on PubMed
Search for more literature for 2.4.2.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.135
-
 phosphate reaction with 7-methylinosine Escherichia coli
0.167
-
 phosphate reaction with 7-methylinosine Cellulomonas sp.
0.169
-
 7-Methylinosine
-
 Cellulomonas sp.
0.247
-
 7-Methylinosine
-
 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
168000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Cellulomonas sp.
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methylinosine + phosphate
-
 Escherichia coli 7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
7-methylinosine + phosphate
-
 Cellulomonas sp. 7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Cellulomonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 10 min, stable Cellulomonas sp.
55
-
 10 min, 20% loss of activity Escherichia coli
60
-
 10 min, about 10% loss of activity Cellulomonas sp.
60
-
 10 min, about 35% loss of activity Escherichia coli
67
-
 10 min, about 90% loss of activity Escherichia coli
67
-
 10 min, 45% loss of activity Cellulomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.01
-
 phosphate reaction with 7-methylinosine Escherichia coli
2.57
-
 7-Methylinosine
-
 Escherichia coli
27.5
-
 7-Methylinosine
-
 Cellulomonas sp.
27.8
-
 phosphate reaction with 7-methylinosine Cellulomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Cellulomonas sp.
8.8 9.3
-
 Escherichia coli