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Literature summary for 2.4.2.1 extracted from
- Purification and properties of inosine-guanosine phosphorylase from Escherichia coli K-12 (1988), J. Bacteriol., 170, 3493-3498.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | - |
Escherichia coli |  |
| deoxyadenosine | - |
Escherichia coli | |
| hypoxanthine arabinoside | - |
Escherichia coli | |
| PCMB | dithiothreitol restores activity | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0033 | - |
hypoxanthine | - |
Escherichia coli | |
| 0.0041 | - |
guanosine | - |
Escherichia coli | |
| 0.044 | - |
deoxyguanosine | - |
Escherichia coli | |
| 0.051 | - |
Xanthosine | - |
Escherichia coli | |
| 0.059 | - |
alpha-D-ribose 1-phosphate | - |
Escherichia coli | |
| 0.062 | - |
2'-deoxyinosine | - |
Escherichia coli | |
| 0.11 | - |
guanosine | - |
Escherichia coli | |
| 0.34 | - |
Inosine | - |
Escherichia coli | |
| 0.6 | - |
2'-deoxyinosine | - |
Escherichia coli | |
| 0.76 | - |
phosphate | - |
Escherichia coli | |
| 2.6 | - |
2',3'-dideoxyinosine | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12, inosine-guanosine phosphorylase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0141 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2',3'-dideoxyinosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-2,3-dideoxyribose 1-phosphate | - |
? | |
| 2'-deoxyinosine + phosphate | - |
Escherichia coli | hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| adenosine + phosphate | no activity | Escherichia coli | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | - |
Escherichia coli | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyinosine + phosphate | 2'-deoxyinosine and 5'-deoxyinosine | Escherichia coli | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| guanosine + phosphate | - |
Escherichia coli | guanine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| xanthosine + phosphate | - |
Escherichia coli | alpha-D-ribose 1-phosphate + xanthine | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
half-life at pH 6-7: 8-9 min. Half-life at pH 7-8: 5-7 min. At pH 6.5 in presence of 5 mg/ml bovine serum albumin half-life is 17 min | Escherichia coli |
| 50 | - |
5 min, 75-85% loss of activity, 1 mM hypoxanthine significantly protects against thermal denaturation | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
phosphorolysis | Escherichia coli |
| 6.8 | - |
synthetic direction | Escherichia coli |
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