Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | - |
Escherichia coli | |
deoxyadenosine | - |
Escherichia coli | |
hypoxanthine arabinoside | - |
Escherichia coli | |
PCMB | dithiothreitol restores activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
hypoxanthine | - |
Escherichia coli | |
0.0041 | - |
guanosine | - |
Escherichia coli | |
0.044 | - |
deoxyguanosine | - |
Escherichia coli | |
0.051 | - |
Xanthosine | - |
Escherichia coli | |
0.059 | - |
alpha-D-ribose 1-phosphate | - |
Escherichia coli | |
0.062 | - |
2'-deoxyinosine | - |
Escherichia coli | |
0.11 | - |
guanosine | - |
Escherichia coli | |
0.34 | - |
Inosine | - |
Escherichia coli | |
0.6 | - |
2'-deoxyinosine | - |
Escherichia coli | |
0.76 | - |
phosphate | - |
Escherichia coli | |
2.6 | - |
2',3'-dideoxyinosine | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12, inosine-guanosine phosphorylase | - |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0141 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2',3'-dideoxyinosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-2,3-dideoxyribose 1-phosphate | - |
? | |
2'-deoxyinosine + phosphate | - |
Escherichia coli | hypoxanthine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | no activity | Escherichia coli | adenine + alpha-D-ribose 1-phosphate | - |
? | |
deoxyguanosine + phosphate | - |
Escherichia coli | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
deoxyinosine + phosphate | 2'-deoxyinosine and 5'-deoxyinosine | Escherichia coli | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
guanosine + phosphate | - |
Escherichia coli | guanine + alpha-D-ribose 1-phosphate | - |
r | |
inosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
xanthosine + phosphate | - |
Escherichia coli | alpha-D-ribose 1-phosphate + xanthine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
half-life at pH 6-7: 8-9 min. Half-life at pH 7-8: 5-7 min. At pH 6.5 in presence of 5 mg/ml bovine serum albumin half-life is 17 min | Escherichia coli |
50 | - |
5 min, 75-85% loss of activity, 1 mM hypoxanthine significantly protects against thermal denaturation | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
phosphorolysis | Escherichia coli |
6.8 | - |
synthetic direction | Escherichia coli |