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Literature summary for 2.4.2.1 extracted from
- Monomeric purine nucleoside phosphorylase from rabbit liver. Purification and characterization (1976), J. Biol. Chem., 251, 407-413.
General Stability
| General Stability | Organism |
|---|---|
| freezing of the solubilized enzyme, especially in dilute solutions, causes complete loss of activity | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-ribose 1-phosphate | - |
Oryctolagus cuniculus |  |
| guanine | - |
Oryctolagus cuniculus | |
| hypoxanthine | - |
Oryctolagus cuniculus | |
| PCMB | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0182 | - |
guanine | - |
Oryctolagus cuniculus | |
| 0.05 | - |
Inosine | - |
Oryctolagus cuniculus | |
| 0.1 | - |
deoxyguanosine | - |
Oryctolagus cuniculus | |
| 0.133 | - |
Inosine | - |
Oryctolagus cuniculus | |
| 0.134 | - |
alpha-D-ribose 1-phosphate | - |
Oryctolagus cuniculus | |
| 15.4 | - |
phosphate | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
x * 39000, SDS-PAGE | Oryctolagus cuniculus |
| 45000 | - |
gel filtration | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| highly susceptible to photooxidation in presence of methylene blue, maximal photoinactivation near pH 8.5 | Oryctolagus cuniculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate | sequential bireactant mechanism | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 47.12 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, enzyme containing 2-mercaptoethanol prior to precipitation with ammonium sulfate, near pH 7, retains maximal catalytic activity for several months when stored as a precipitate | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyguanosine + phosphate | - |
Oryctolagus cuniculus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Oryctolagus cuniculus | guanine + alpha-D-ribose 1-phosphate | - |
r | |
| inosine + phosphate | - |
Oryctolagus cuniculus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| xanthosine + phosphate | reaction only at high concentrations | Oryctolagus cuniculus | alpha-D-ribose 1-phosphate + xanthine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 39000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
20 min, 10% loss of activity | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Oryctolagus cuniculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.361 | - |
alpha-D-ribose 1-phosphate | - |
Oryctolagus cuniculus | |
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