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Literature summary for 2.4.2.1 extracted from
- Purine nucleoside phosphorylase from human erythrocytes (1978), Methods Enzymol., 51, 530-538.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Homo sapiens |  |
| PCMB | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.019 | - |
hypoxanthine | - |
Homo sapiens | |
| 0.032 | - |
phosphate | - |
Homo sapiens | |
| 0.047 | - |
guanosine | - |
Homo sapiens | |
| 0.048 | - |
Inosine | - |
Homo sapiens | |
| 0.066 | - |
deoxyinosine | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
x * 30000, SDS-PAGE | Homo sapiens |
| 80000 | 92000 | gel filtration, equilibrium sedimentation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| freezing of the enzyme in presence of dithiothreitol results in greater loss of activity than in the absence of a sulfhydryl reagent | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate | ordered bi-bi mechanism with nucleoside being the first substrate to add and base product to leave | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 93 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 20°C, room temperature, stable for several days in concentrated solution, 1% in 0.02% sodium azide | Homo sapiens |
| 4°C or frozen, stable for long periods of time | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyinosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| guanosine + phosphate | - |
Homo sapiens | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Homo sapiens | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30000, SDS-PAGE | Homo sapiens |
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Release 2023.1 (January 2023)
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