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Literature summary for 2.4.2.1 extracted from
- Purification and characterization of second thermostable purine nucleoside phosphorylase in Bacillus stearothermophilus JTS 859 (1989), Agric. Biol. Chem., 53, 3219-3224.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PCMB | no inhibition of PUNPII | Geobacillus stearothermophilus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.075 | - |
adenosine | - |
Geobacillus stearothermophilus | |
| 0.19 | - |
2'-deoxyadenosine | - |
Geobacillus stearothermophilus | |
| 0.22 | - |
Inosine | - |
Geobacillus stearothermophilus | |
| 0.34 | - |
guanosine | - |
Geobacillus stearothermophilus | |
| 0.92 | - |
2'-deoxyinosine | - |
Geobacillus stearothermophilus | |
| 1.9 | - |
2'-deoxyguanosine | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 113000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
JTS 859 | - |
| Geobacillus stearothermophilus JTS 859 | - |
JTS 859 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| PUNPI and PUNPII | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 50.6 | - |
PUNPII | Geobacillus stearothermophilus |
| 405 | - |
PUNPI | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + phosphate | 160% of the activity with inosine | Geobacillus stearothermophilus | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| adenosine + phosphate | activity with OUNOII, no activity with PUNPI | Geobacillus stearothermophilus | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| adenosine + phosphate | 160% of the activity with inosine | Geobacillus stearothermophilus JTS 859 | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| deoxyadenosine + phosphate | 2'-deoxyadenosine, 160% of the activity with inosine | Geobacillus stearothermophilus | alpha-D-ribose 1-phosphate + adenine | - |
? | |
| deoxyguanosine + phosphate | 2'-deoxyguanosine, 469% of the activity with inosine | Geobacillus stearothermophilus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | 2'-deoxyguanosine, 469% of the activity with inosine | Geobacillus stearothermophilus JTS 859 | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyinosine + phosphate | 2'-deoxyinosine, 691% of the activity with inosine | Geobacillus stearothermophilus | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| deoxyinosine + phosphate | 2'-deoxyinosine, 691% of the activity with inosine | Geobacillus stearothermophilus JTS 859 | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| guanosine + phosphate | 53% of the activity with inosine | Geobacillus stearothermophilus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | 53% of the activity with inosine | Geobacillus stearothermophilus JTS 859 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Geobacillus stearothermophilus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Geobacillus stearothermophilus JTS 859 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life of isoenzyme PUNPII is 15 min | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | - |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11.5 | pH 5.0: about 50% of maximal activity, pH 11.5: about 85% of maximal activity | Geobacillus stearothermophilus |
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