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Literature summary for 2.4.2.1 extracted from

  • Shirae, H.; Yokozeki, K.
    Purifications and properties of orotidine-phosphorolyzing enzyme and purine nucleoside phosphorylase from Erwinia carotovora AJ 2992 (1991), Agric. Biol. Chem., 55, 1849-1857.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Pectobacterium carotovorum
CuSO4
-
Pectobacterium carotovorum
HgCl2
-
Pectobacterium carotovorum
PCMB
-
Pectobacterium carotovorum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.85
-
guanosine at 40°C Pectobacterium carotovorum
1.92
-
Inosine at 40°C Pectobacterium carotovorum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
non-denaturing PAGE Pectobacterium carotovorum

Organism

Organism UniProt Comment Textmining
Pectobacterium carotovorum
-
AJ 2992
-
Pectobacterium carotovorum AJ 2992
-
AJ 2992
-

Purification (Commentary)

Purification (Comment) Organism
-
Pectobacterium carotovorum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
48.5
-
-
Pectobacterium carotovorum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxyinosine + phosphate 12% of the activity with inosine Pectobacterium carotovorum alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine
-
?
adenosine + phosphate 48% of the activity with inosine Pectobacterium carotovorum adenine + alpha-D-ribose 1-phosphate
-
?
adenosine + phosphate 48% of the activity with inosine Pectobacterium carotovorum AJ 2992 adenine + alpha-D-ribose 1-phosphate
-
?
deoxyadenosine + phosphate 2'-deoxyadenosine, 57% of the activity with inosine. 3'-deoxyadenosine, 7% of the activity with inosine Pectobacterium carotovorum alpha-D-ribose 1-phosphate + adenine
-
?
deoxyguanosine + phosphate 2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine Pectobacterium carotovorum guanine + alpha-D-deoxyribose 1-phosphate
-
?
deoxyguanosine + phosphate 2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine Pectobacterium carotovorum AJ 2992 guanine + alpha-D-deoxyribose 1-phosphate
-
?
deoxyinosine + phosphate 136% of the activity with inosine Pectobacterium carotovorum hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
r
deoxyinosine + phosphate 136% of the activity with inosine Pectobacterium carotovorum AJ 2992 hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
r
guanosine + phosphate 2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine Pectobacterium carotovorum guanine + alpha-D-ribose 1-phosphate
-
?
guanosine + phosphate 2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine Pectobacterium carotovorum AJ 2992 guanine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Pectobacterium carotovorum hypoxanthine + alpha-D-ribose 1-phosphate
-
?
inosine + phosphate
-
Pectobacterium carotovorum AJ 2992 hypoxanthine + alpha-D-ribose 1-phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 58000, SDS-PAGE Pectobacterium carotovorum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Pectobacterium carotovorum
70
-
-
Pectobacterium carotovorum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Pectobacterium carotovorum