Any feedback?
Literature summary for 2.4.1.91 extracted from
- Cloning and characterization of Vitis vinifera UDP-glucose:flavonoid 3-O-glucosyltransferase, a homolog of the enzyme encoded by the maize bronze-1 locus that may primarily serve to glucosylate anthocyanidins in vivo (1998), J. Biol. Chem., 273, 9224-9233.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Vitis vinifera |
General Stability
| General Stability | Organism |
|---|---|
| at least three freeze-thaw cycles can be tolerated without apparent loss of activity | Vitis vinifera |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Vitis vinifera |  |
| Mg2+ | - |
Vitis vinifera | |
| Zn2+ | - |
Vitis vinifera | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
quercetin | recombinant enzyme | Vitis vinifera | |
| 0.016 | - |
delphinidin | recombinant enzyme | Vitis vinifera | |
| 0.03 | - |
cyanidin | recombinant enzyme | Vitis vinifera | |
| 0.0357 | - |
malvidin | recombinant enzyme | Vitis vinifera | |
| 1.9 | - |
UDPglucose | recombinant enzyme | Vitis vinifera | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + a flavonol | Vitis vinifera | the principal, if not only, role of the enzyme is to glucosylate anthocyanidins in red fruit during ripening | UDP + a flavonol 3-O-D-glucoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vitis vinifera | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Vitis vinifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Vitis vinifera | - |
| leaf | - |
Vitis vinifera | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or 4°C, the recombinant enzyme is stable for at least 120 h | Vitis vinifera |
| -80°C, stable for many months | Vitis vinifera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | only UDPglucose can serve as glucosyl donor | Vitis vinifera | ? | - |
? | |
| UDP-glucose + delphinidin | - |
Vitis vinifera | UDP + delphinidin 3-O-glucoside | - |
? | |
| UDPglucose + a flavonol | the principal, if not only, role of the enzyme is to glucosylate anthocyanidins in red fruit during ripening | Vitis vinifera | UDP + a flavonol 3-O-D-glucoside | - |
? | |
| UDPglucose + cyanidin | - |
Vitis vinifera | UDP + cyanidin 3-O-glucoside | - |
? | |
| UDPglucose + malvidin | - |
Vitis vinifera | UDP + malvidin 3-O-glucoside | - |
? | |
| UDPglucose + quercetin | - |
Vitis vinifera | UDP + quercetin 3-O-glucoside | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
reaction with quercetin or cyanidin | Vitis vinifera |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
